Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 May 2;588(9):1787-94.
doi: 10.1016/j.febslet.2014.03.039. Epub 2014 Mar 28.

Effects of PipX on NtcA-dependent promoters and characterization of the cox3 promoter region in the heterocyst-forming cyanobacterium Anabaena sp. PCC 7120

Sergio Camargo  1 , Ana Valladares  1 , Karl Forchhammer  2 , Antonia Herrero  3
Affiliations
Free article

Effects of PipX on NtcA-dependent promoters and characterization of the cox3 promoter region in the heterocyst-forming cyanobacterium Anabaena sp. PCC 7120

Sergio Camargo et al. FEBS Lett. .
Free article

Abstract

In Anabaena, the pipX gene is induced in the cells differentiating into heterocysts, being the PipX factor required for full expression of late heterocyst-specific genes. Here we show that PipX has a positive effect on in vitro binding of the transcription factor NtcA to DNA, as well as on transcript production, in different NtcA-dependent promoters. We found that the cox3 operon, encoding a heterocyst-specific terminal respiratory oxidase, is expressed from three nitrogen-regulated promoters to which NtcA binds. At the three sites, NtcA binding is potentiated by PipX. Thus, PipX has a direct effect on gene expression influencing the activity of NtcA.

Keywords: Cyanobacteria; Transcriptional regulation.

PubMed Disclaimer

Publication types

LinkOut - more resources