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. 2014 May:407:35-9.
doi: 10.1016/j.jim.2014.03.016. Epub 2014 Mar 28.

Using fluorescence correlation spectroscopy (FCS) for IFN-g detection: a preliminary study

Chen Shen  1 , Martin Knapp  2 , Martin Gerald Puchinger  2 , Aamir Shahzad  2 , Erwin Gaubitzer  2 , A Dong Shen  3 , Gottfried Koehler  2
Affiliations

Using fluorescence correlation spectroscopy (FCS) for IFN-g detection: a preliminary study

Chen Shen et al. J Immunol Methods. 2014 May.

Abstract

Nowadays, enzyme-linked immunosorbent assay (ELISA) based detection of Mycobacterium tuberculosis (M. tuberculosis) antigen triggered interferon-gamma (IFN-g) secretion by blood T cells displays an improved diagnostic value for M. tuberculosis infection. Applications of fluorescence correlation spectroscopy (FCS) have been explored in various subfields of medicine and molecular biology, including detection of a certain biomarker in liquid instead of ELISA. Here, we present a preliminary study of detecting IFN-g using FCS-based technique.

Keywords: Diagnostic; Fluorescence correlation spectroscopy; Interferon-gamma; Monoclonal antibodies.

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