Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Jan;80(1):65-8.
doi: 10.1111/j.1349-7006.1989.tb02246.x.

Inhibition of intracellular bleomycin hydrolase activity by E-64 leads to the potentiation of the cytotoxicity of peplomycin against Chinese hamster lung cells

C Nishimura  1 , T Nishimura, N Tanaka, H Yamaguchi, H Suzuki
Affiliations

Inhibition of intracellular bleomycin hydrolase activity by E-64 leads to the potentiation of the cytotoxicity of peplomycin against Chinese hamster lung cells

C Nishimura et al. Jpn J Cancer Res. 1989 Jan.

Abstract

N-(N-(L-3-trans-carboxyoxiran-2-carbonyl)-L-leucyl)-agmatine (E-64), a thiol protease inhibitor, potentiated the cytotoxicity of peplomycin against the Chinese hamster lung (V79) cell. After the treatment of the cells with E-64 (50 micrograms/ml) for 12 h, bleomycin hydrolase activity of the cells was almost completely inhibited. V79 cells treated with [3H]peplomycin for 24 h in the presence of E-64 (50 micrograms/ml) accumulated twice as much [3H]peplomycin and five times less [3H]desamidopeplomycin compared with V79 cells treated in the absence of E-64. These results suggest that E-64 increases the sensitivity of V79 cells to peplomycin probably by inhibiting the intracellular bleomycin hydrolase activity.

PubMed Disclaimer

References

    1. ) Umezawa , H. , Takeuchi , T. , Hori , S. , Sawa , T. , Ishizuka , M. , Ichikawa , T. and Komai , T.Studies on the mechanism of antitumor effect of bleomycin on squamous cell carcinoma . J. Antibiot. , 25 , 409 – 420 ( 1972. ). - PubMed
    1. ) Sugiura , Y. , Muraoka , Y. , Fujii , A. , Takita , T. and Umezawa , H.Chemistry of bleomycin. XXIV. Desamido bleomycin from viewpoint of metal coordination and oxygen activation . J. Antibiot. , 32 , 756 – 758 ( 1979. ). - PubMed
    1. ) Nishimura , C. , Tanaka , N. , Suzuki , H. and Tanaka , N.Purification of bleomycin hydrolase with a monoclonal antibody and its characterization . Biochemistry , 26 , 1574 – 1578 ( 1987. ). - PubMed
    1. ) Sebti , S. M. , De Leon , J. C. and Lazo , J. S.Purification, characterization, and amino acid composition of rabbit pulmonary bleomycin hydrolase . Biochemistry , 26 , 4213 – 4219 ( 1987. ). - PubMed
    1. ) Hanada , K. , Tamai , M. , Yamagishi , M. , Ohmura , S. , Sawada , J. and Tanaka , I.Isolation and characterization of E‐64, a new thiol protease inhibitor . Agric. Biol. Chem. , 42 , 523 – 528 ( 1978. ).

Publication types

LinkOut - more resources