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. 2014 Jun;58(6):3538-40.
doi: 10.1128/AAC.02793-13. Epub 2014 Mar 31.

Biochemical analysis of metallo-β-lactamase NDM-3 from a multidrug-resistant Escherichia coli strain isolated in Japan

Tatsuya Tada  1 , Tohru Miyoshi-Akiyama  1 , Kayo Shimada  1 , Teruo Kirikae  2
Affiliations

Biochemical analysis of metallo-β-lactamase NDM-3 from a multidrug-resistant Escherichia coli strain isolated in Japan

Tatsuya Tada et al. Antimicrob Agents Chemother. 2014 Jun.

Abstract

New Delhi metallo-β-lactamase-3 (NDM-3) was identified in a multidrug-resistant Escherichia coli isolate, NCGM77, obtained from the feces of a patient in Japan. The enzymatic activities of NDM-3 against β-lactams were similar to those of NDM-1, although NDM-3 showed slightly lower kcat/Km ratios for all the β-lactams tested except for doripenem. The genetic context for blaNDM-3 was tnpA-blaNDM-3-bleMBL-trpF-dsbC-tnpA-sulI-qacEdeltaI-aadA2-dfrA1, which was present on an approximately 250-kb plasmid.

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Figures

FIG 1
FIG 1
Localization of the blaNDM-3 gene on the plasmid of E. coli strain NCGM77 separated by PFGE. Lane M, midrange PFG marker (New England BioLabs, Tokyo, Japan); lane A, plasmids of E. coli strain NCGM77; lane B, hybridization of the plasmid with a probe specific for the blaNDM-3 gene.
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