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. 2014 Mar;37(1):54-60.
doi: 10.1590/s1415-47572014000100010. Epub 2013 Feb 28.

Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil

Elizabete Cristina da Silva  1 , Concepta Margaret McManus  2 , Maria Pia Souza Lima Mattos de Paiva Guimarães  3 , Aurora M G Gouveia  4 , Olivardo Facó  5 , Daniel M Pimentel  6 , Alexandre Rodrigues Caetano  1 , Samuel Rezende Paiva  7
Affiliations

Validation of a microsatellite panel for parentage testing of locally adapted and commercial goats in Brazil

Elizabete Cristina da Silva et al. Genet Mol Biol. 2014 Mar.

Abstract

Brazilian goats are generally kept in small herds and extensive rearing systems, mainly in the northeastern region of the country. Despite production improvement in recent years, the lack of pedigree control has affected genetic progress. This study aimed to validate a panel of 16 microsatellites for parentage testing in locally adapted and commercial goats breeds raised in Brazil, as well as to compare its efficiency with the panel recommended by the Brazilian Ministry of Agriculture, Livestock and Supplies (MAPA) in 2004. The number of alleles and expected heterozygosity (He) per marker ranged from four to 18, and from 0.051 to 0.831, respectively. Using all markers, 100% of parentage cases of the validation dataset were resolved with a strict confidence level of 95%. The 16 microsatellites panel showed adequate exclusion power (99.99%) and identity accuracy (99.99%). Suggestions for improvement of the marker panel endorsed by MAPA are provided.

Keywords: Capra hircus; animal breeding; animal genetic resources; parentage errors.

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