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Review
. 2013 Aug 24:8:e201308003.
doi: 10.5936/csbj.201308003. eCollection 2013.

A Sieve-Raft Hypothesis for the regulation of endothelial fenestrations

Victoria C Cogger  1 , Ute Roessner  2 , Alessandra Warren  1 , Robin Fraser  3 , David G Le Couteur  1
Affiliations
Review

A Sieve-Raft Hypothesis for the regulation of endothelial fenestrations

Victoria C Cogger et al. Comput Struct Biotechnol J. .
No abstract available

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Figures

Figure 1
Figure 1
Microscopy of LSEC fenestrations and the LSEC membrane. Figure 1A is a scanning electron micrograph of an isolated LSEC in culture. The micrograph clearly displays fenestrations, examples are denoted by an asterix (*), arranged in groups (sieve plates) or individually. The fenestrations are located in the thin cytoplasmic extensions of the cell, distal to the nucleus (N) Scale bar = 5 µm. Figure 1B is a transmission electron micrograph of perfusion fixed liver, the unique architecture of the sinusoid can be seen. The very thin endothelium (E) is perforated with fenestrations (#), allowing passage of substrates into the hepatocytes (H) for metabolism, storage and detoxification Scale bar = 2 µm. Figure 1C is a micrograph prepared by 3D structured illumination microscopy. The LSECs have been stained with Bodipy FL C5 ganglioside GM1, a marker for rafts (green) and Cell-Mask Orange, a cell membrane marker (orange). There is an inverse distribution between liver sieve plates and membrane rafts. Some sieve plates are identified by an arrow (→) and fenestrations can be resolved within the sieve plates. Scale bar = 1 µm.
Figure 2
Figure 2
The Sieve-Raft hypothesis: the composition and arrangement of lipids in the cell membrane is paramount in determining fenestration formation and cell function. We propose that fenestrations form in non-raft microdomains of the lipid bilayer and that rafts and actin engender membrane stability, while limiting fenestration formation.
See this image and copyright information in PMC

References

    1. Hilmer, SN, Cogger VC, Fraser R, McLean AJ, Sullivan D and Le Couteur DG, Age-related changes in the hepatic sinusoidal endothelium impede lipoprotein transfer in the rat. Hepatology, 2005. 42(6): p. 1349–1354 - PubMed
    1. Pradel, G and Frevert U, Malaria sporozoites actively enter and pass through rat Kupffer cells prior to hepatocyte invasion. Hepatology, 2001. 33(5): p. 1154–1165 - PubMed
    1. Mitchell, SJ, Huizer-Pajkos A, Cogger VC, McLachlan AJ, Le Couteur DG, Jones B, de Cabo R, Hilmer SN, Age-related pseudocapillarization of the liver sinusoidal endothelium impairs the hepatic clearance of acetaminophen in rats. J Gerontol A Biol Sci Med Sci, 2011. 66(4): p. 400–408 - PMC - PubMed
    1. Fraser, RLe Couteur DGWarren ACogger VC, Bertolino P and Smith M. The liver sieve and gene therapy. Blood,, 101(8): p. 3338; author reply 3338-3339 - PubMed
    1. Mueller, T, Rumpel E, Hradetzky S, Bollig F, Wegner H, Blumenthal A, Greinacher A, Endlich K, Endlich N. 2011. Non-muscle myosin IIA is required for the development of the zebrafish glomerulus. Kidney International, 2003. 80(10): p. 1055–1063 - PubMed

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