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. 2014:1138:377-90.
doi: 10.1007/978-1-4939-0348-1_23.

Animal models in dengue

Emily Plummer  1 , Sujan Shresta
Affiliations

Animal models in dengue

Emily Plummer et al. Methods Mol Biol. 2014.

Abstract

Validation of a mouse model of dengue virus (DENV) infection relies on verification of viremia and productive replication in mouse tissues following infection. Here, we describe a quantitative assay for determining viral RNA levels in mouse serum and tissues. For the purpose of confirming DENV replication, we outline a fluorescence immunohistochemistry (FIHC) protocol for staining a nonstructural protein of DENV.

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