Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Jul;30(7):1191-6.
doi: 10.1007/s00381-014-2409-4. Epub 2014 Apr 4.

Circulating proteasome activity following mild head injury in children

Marzena Tylicka  1 , Ewa Matuszczak, Wojciech Dębek, Adam Hermanowicz, Halina Ostrowska
Affiliations

Circulating proteasome activity following mild head injury in children

Marzena Tylicka et al. Childs Nerv Syst. 2014 Jul.

Abstract

Purpose: The aim of the study is to characterize changes in circulating proteasome (c-proteasome) activity following mild traumatic brain injury in children.

Methods: Fifty children managed at the Department of Pediatric Surgery because of concussion-mild head injury was randomly included into the study. The children were aged 11 months to 17 years (median = 10.07 + -1.91 years). Plasma proteasome activity was assessed using Suc-Leu-Leu-Val-Tyr-AMC peptide substrate, 2-6 h, 12-16 h, and 2 days after injury. Twenty healthy children admitted for planned inguinal hernia repair served as controls.

Results: Statistically significant elevation of plasma c-proteasome activity was noted in children with mild head injury 2-6 h, 12-16 h, and 2 days after the injury.

Conclusions: Authors observed a statistically significant upward trend in the c-proteasome activity between 2-6 and 12-16 h after the mild head injury, consistent with the onset of the symptoms of cerebral concussion and a downward trend in the c-proteasome activity in the plasma of children with mild head injury between 12-16 h and on the second day after the injury, consistent with the resolving of the symptoms of cerebral concussion. Further studies are needed to demonstrate that the proteasome activity could be a prognostic factor, which can help in further diagnostic and therapeutic decisions in patients with head injury.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Circulating proteasome activity in the plasma of children, 2–6 h (1, n = 50), 12–16 h (2, n = 45), 2 days (3, n = 41) after mild head injury and in control group
See this image and copyright information in PMC

References

    1. Chambellan A, Cruickshank PJ, McKenzie P, Cannady SB, Szabo K, Comhair SA, Erzurum SC. Gene expression profile of human airway epithelium induced by hyperoxia in vivo. Am J Res Cell Mol Biol. 2006;35:424–435. doi: 10.1165/rcmb.2005-0251OC. - DOI - PMC - PubMed
    1. Davies KJ. Degradation of oxidized proteins by the 20S proteasome. Biochimie. 2001;83:301–310. doi: 10.1016/S0300-9084(01)01250-0. - DOI - PubMed
    1. Dawson TM, Dawson VL. Molecular pathways of neurodegeneration in Parkinson’s disease. Science. 2003;302:819–822. doi: 10.1126/science.1087753. - DOI - PubMed
    1. Diaz-Hernandez M, Hernandez F, Martin-Aparicio E, Gomez-Ramos P, Moran MA, Castano JG, Ferrer I, Avila J, Lucas JJ. Neuronal induction of the immunoproteasome in Huntington’s disease. J Neurosci. 2003;23:11653–11661. - PMC - PubMed
    1. Dutaud D, Aubry L, Henry L, Levieux D, Hendil KB, Kuehn L, Bureau JP, Ouali A. Development and evaluation of a sandwich ELISA for quantification of the 20S proteasome in human plasma. J Immunol Methods. 2002;260:183–19. doi: 10.1016/S0022-1759(01)00555-5. - DOI - PubMed

Substances

LinkOut - more resources