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. 2013 Jul;5(2):79-82.
doi: 10.4103/0974-2727.119845.

In silico Analysis of TCR Vβ7 of Two Patients with Type 1 Diabetes Mellitus

Jianwei Zhou  1 , Cui Kong  2 , Xiukui Wang  3 , Yinfeng Jia  1 , Li Wang  4 , Hong Chang  1 , Lin Sun  5
Affiliations

In silico Analysis of TCR Vβ7 of Two Patients with Type 1 Diabetes Mellitus

Jianwei Zhou et al. J Lab Physicians. 2013 Jul.

Abstract

Objective: To compare the sequences and crystal structures of variable region of beta chain 7 (Vβ7) of T cell receptor (TCR) of two patients with type 1 diabetes mellitus (T1DM).

Patients and methods: The skewness of TCR Vβ7 of two T1DM patients were detected with real-time florescence quantitative polymerase chain reaction (FQ-PCR) and deoxyribonucleic acid (DNA) melting curve analysis technique followed by being sequenced, the crystal structures of them were simulated according to CPH models 2.0 Server, IMGT database, and RasMol 2 software.

Results: The whole sequences of TCR Vβ7 of T1DM patient-1 were "CASRTAGQYEQYFGPGTR", that of patient-2 were "CASRTAGQYEQFFGPGTR"; the only difference between them lied on the 12(th) amino acid. The crystal structures of Vβ7 of the two patients simulated with backbone model were rather similar, while that with sphere model were obviously different.

Conclusion: Although the TCR Vβ7 of the T1DM patients share the similar gene sequences, their crystal structures simulated with sphere model are different, and the mechanism needs further study.

Keywords: Beta chain; T cell receptor; crystal structure; type 1 diabetes mellitus; variable region.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
The crystal structures (in backbone model) of T cell receptor of variable region of beta chain 7 (TCR Vβ7) of two type 1 diabetes mellitus (T1DM) patients made by CPH models 2.0 Server and IMGT database. (a) The crystal structure of TCR Vβ7 of patient-1 with T1DM. (b) The crystal structure of TCR Vβ7 of patient-2 with T1DM
Figure 2
Figure 2
The crystal structures (in sphere model) of TCR Vβ7 of two T1DM patients made by CPH models 2.0 Server and RasMol 2 software. (a) The crystal structure of TCR Vβ7 of patient-1 with T1DM. (b) The crystal structure of TCR Vβ7 of patient-2 with T1DM
Figure 3
Figure 3
The predicted antigen peptides specific to T1DM according to crystal structures of TCR Vβ7 of two T1DM patients. (a) The predicted antigen peptides specific to T1DM according to crystal structures of TCR Vβ7 of patient-1 with T1DM. (b) The predicted antigen peptides specific to T1DM according to crystal structures of TCR Vβ7 of patient-2 with T1DM
See this image and copyright information in PMC

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