doi: 10.1126/science.2470151.
Stereochemical course of catalysis by the Tetrahymena ribozyme
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- PMID: 2470151
- DOI: 10.1126/science.2470151
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Stereochemical course of catalysis by the Tetrahymena ribozyme
Science.
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Abstract
The group I intron from Tetrahymena catalyzes phosphodiester transfer reactions on various RNA substrates. A modified RNA substrate with a phosphorothioate group in one stereoisomeric form at the site of reaction was synthesized in order to determine the stereochemical course of an RNA-catalyzed reaction. The reaction product was digested with a stereospecific nuclease to determine the configuration of the product phosphorothioate. The reaction occurs with inversion of configuration at phosphorus, implying an in-line pathway for the reaction.
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