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. 2014 Apr 4;9(4):e92567.
doi: 10.1371/journal.pone.0092567. eCollection 2014.

An integrated genetic and cytogenetic map for Zhikong scallop, Chlamys farreri, based on microsatellite markers

Liying Feng  1 , Liping Hu  2 , Xiaoteng Fu  1 , Huan Liao  1 , Xuan Li  1 , Aibin Zhan  3 , Lingling Zhang  1 , Shi Wang  1 , Xiaoting Huang  1 , Zhenmin Bao  1
Affiliations

An integrated genetic and cytogenetic map for Zhikong scallop, Chlamys farreri, based on microsatellite markers

Liying Feng et al. PLoS One. .

Erratum in

  • PLoS One. 2014;9(7):e103167

Abstract

The reliability of genome analysis and proficiency of genetic manipulation requires knowledge of the correspondence between the genetic and cytogenetic maps. In the present study, we integrated cytogenetic and microsatellite-based linkage maps for Zhikong scallop, Chlamys farreri. Thirty-eight marker-anchored BAC clones standing for the 19 linkage groups were used to be FISH probes. Of 38 BAC clones, 30 were successfully located on single chromosome by FISH and used to integrate the genetic and cytogenetic map. Among the 19 linkage groups, 12 linkage groups were physically anchored by 2 markers, 6 linkage groups were anchored by 1 marker, and one linkage group was not anchored any makers by FISH. In addition, using two-color FISH, six linkage groups were distinguished by different chromosomal location; linkage groups LG6 and LG16 were placed on chromosome 10, LG8 and LG18 on chromosome 14. As a result, 18 of 19 linkage groups were localized to 17 pairs of chromosomes of C. farreri. We first integrated genetic and cytogenetic map for C. farreri. These 30 chromosome specific BAC clones in the cytogenetic map could be used to identify chromosomes of C. farreri. The integrated map will greatly facilitate molecular genetic studies that will be helpful for breeding applications in C. farreri and the upcoming genome projects of this species.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. FISH results of 24 BAC clones from LG1 to LG15 with specific signal on C. farreri chromosomes.
(A1) BH1304, (A2) BH1162H2, (B1) BH783B4, (B2) BH793B11, (C1) BH799B12, (C2) BH1049A9, (D) BB311A9, (E1) BB105A1, (E2) BB86D4, (F) BH1285H8, (G) BH1291D12, (H) BH966F2, (I1) BB224E11, (I2) BB98C5, (J) BB39F11, (K1) BB75B6, (K2) BH1308E3, (L1) BH885H2, (L2) BB224B4, (M1) BB233G7, (M2) BB312B11, (N1) BH984B5, (N2) BB105B2, (O) BB235A11. Bars = 5 µm.
Figure 2
Figure 2. FISH results of 8 BAC clones from LG16, LG17 and LG18 with specific signal on C. farreri chromosomes.
(P1) BH565D6, (P2) BB69B10, (Q1) BH368F12, (Q2) BH1261C3, (R1) BH1003G1, (R2) BH986B2, (S1) BH431C4, (S2) BB239A6. Bars = 5 µm.
Figure 3
Figure 3. FISH results of microsatellite-anchored BAC clones without specific signal on chromosomes.
(A) BB322D4 without C0t-1 DNA blocking, (B) BB322D4 with C0t-1 DNA blocking, (C) BB27B5 with C0t-1 DNA blocking, (D) BH1060D10 with C0t-1 DNA blocking. Bars = 5 µm.
Figure 4
Figure 4. Two-color FISH results of the different BAC clones.
(A) LG1, BH1304E11 (Red) and BH1162H2 (Green);(B) LG2, BH783B4 (Green) and BH793B11 (Red); (C) LG3, BH799B12 (Green) and BH1049A9 (Green);(D) LG5, BB105A1 (Red) and BB86D4 (Green);(E) LG11, BB75B6 (Red) and BH1308E3 (Green);(F) LG12, BH885H2 (Red) and BB224B4 (Green);(G) LG14, BH984B5 (Green) and BB105B2 (Red);(H) LG17, BH368F12 (Red) and BH1261C3 (Green). Bars = 5 µm.
Figure 5
Figure 5. Integration of the 18 genetic linkage groups with individual chromosomes of Zhikong scallop, C. farreri.
Figure 6
Figure 6. Two-color FISH results of the different linkage groups.
(A) LG13, Chromosome 6, BB233G7 (Red) and Chromosome 6, F458F11 (Green); (B) LG1, Chromosome 8, BH1304E11 (Red, arrow head), LG3, Chromosome 7, BH1049A9 (Red, arrow) and Chromosome 6, F458F11 (Green); (C) LG3, Chromosome 7, BH1049A9 (Red) and LG18, Chromosome 11, BH1003G1 (Green); (D) LG16, Chromosome 10, BB69B10 (Red) and Chromosome 10, F408A12 (Green); (E) LG1, Chromosome 8, BH1304E11(Red) and Chromosome 10, F408A12 (Green); (F) LG18, Chromosome 11, BH1003G1 (Red) and Chromosome 10, F408A12 (Green); (G) LG7, Chromosome 13, BH1291D12 (Red) and Chromosome 10, F408A12 (Green); (H) LG7, Chromosome 13, BH1291D12 (Green) and LG18, Chromosome 11, BH1003G1 (Red); (I) LG6, BH1285H8 (Red) and LG16 BB69B10 (Green); (J) LG8, BH966F2 (Red) and LG18, BH986B2 (Green). Bars = 5 µm.
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