Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Mar-Apr;20(2):108-12.
doi: 10.4103/1319-3767.129475.

TNF-alpha gene polymorphisms in Iranian Azari Turkish patients with inflammatory bowel diseases

Mortaza Bonyadi  1 , Reza Abdolmohammadi, Zohreh Jahanafrooz, Mohammad-Hosein Somy, Manoochehr Khoshbaten
Affiliations

TNF-alpha gene polymorphisms in Iranian Azari Turkish patients with inflammatory bowel diseases

Mortaza Bonyadi et al. Saudi J Gastroenterol. 2014 Mar-Apr.

Abstract

Context: Crohn's disease (CD) and ulcerative colitis (UC) are chronic inflammatory diseases of the bowel (IBD) whose causes are not fully known. Emerging data indicate that alterations in cytokine synthesis may play a role in IBD pathogenesis.

Aims: We aimed to determine the association between tumor necrosis factor-alfa (TNFα) promoter polymorphisms (at positions - 308 and - 1031) and susceptibility to IBD among Iranian Azari Turkish patients.

Settings and design: One hundred and one patients with IBD and 100 healthy subjects were analyzed.

Materials and methods: Both polymorphisms in the promoter region of the TNFα gene at positions -1031T/C and -308G/A were detected by polymerase chain reaction-restriction fragment length polymorphism assay. All statistical analyses were calculated with SPSS for Windows 16.0. The Fisher's exact test was used to test for departure from Hardy-Weinberg equilibrium of the genotype frequencies (P > 0.05).

Results: The allele frequency of the TNFα-308G and -1031T were higher in IBD patients but did not reach statistical significance. However, the homozygous TT genotype for the SNP-1031 T > C was significantly higher in UC patients than in healthy controls (P = 0.01) and the heterozygous CT genotype for the SNP -1031 T > C was significantly lower in UC patients than in healthy controls (P = 0.03).

Conclusions: The TNFα-1031 T allele confers a significant risk for developing UC in Iranian Azeri Turkish patients. Also the frequency of TNFα-1031 C allele was considerably low among patients with UC and it may have protective role among them (OR = 0.43; P = 0.01).

PubMed Disclaimer

Conflict of interest statement

Conflict of Interest: None declared.

References

    1. Ferguson L, Huebner C, Peterman I, Gearry R, Barclay M, Demmers P, et al. Han Single nucleotide polymorphism in the tumor necrosis factor-alpha gene affects inflammatory bowel diseases risk. World J Gastroenterol. 2008;14:4652–61. - PMC - PubMed
    1. Balding J, Livingstone W, Conroy J, Mynett-Johnson L, Weir D, Mahmud N, et al. Inflammatory bowel disease: The role of inflammatory cytokine gene polymorphisms. Mediators of Inflamm. 2004;13:181–7. - PMC - PubMed
    1. Baumgart D. The diagnosis and treatment of Crohn's disease and ulcerative colitis. Dtsch Arztebl Int. 2009;106:123–33. - PMC - PubMed
    1. Fan W, Maoqing W, Wang Yang Ch, Fulan H, Dandan L, Jiaojiao R, et al. Relationship between the polymorphism of tumor necrosis factor-α-308 G4A and susceptibility to inflammatory bowel diseases and colorectal cancer: A meta-analysis. Eur J Hum Genet. 2011;19:432–7. - PMC - PubMed
    1. Van Heel D, Udalova I, De Silva A, McGovern D, Kinouchi Y, Hull J, et al. Inflammatory bowel disease is associated with a TNF polymorphism that affects an interaction between the OCT1 and NF-κB transcription factors. Hum Mol Genet. 2002;11:1281–9. - PubMed

Publication types

Substances

LinkOut - more resources