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. 1989 Apr 25;17(8):3057-63.
doi: 10.1093/nar/17.8.3057.

Polyadenylated H3 histone transcripts and H3 histone variants in alfalfa

S C Wu  1 , J Györgyey, D Dudits
Affiliations
Free PMC article

Polyadenylated H3 histone transcripts and H3 histone variants in alfalfa

S C Wu et al. Nucleic Acids Res. .
Free PMC article

Abstract

Histone H3 mRNAs were found in polyA(+) fractions of total RNA prepared from alfalfa plants, calli and somatic embryos. The sequence analysis of cDNAs revealed the presence of a polyA tail on independent alfalfa H3 mRNAs. A highly conserved sequence motif AAUGAAA identified about 20bp upstream from the 3' ends of the alfalfa H3 cDNAs was suggested to be one of the possible regulatory elements in the 3' end formation and polyadenylation. Three out of the four analysed H3 cDNAs have more than 97% homology with a genomic clone and encode the same protein. While the fourth represents a minor species with only 78.8% homology to the coding region of the genomic clone and encodes a H3 histone with four amino acid replacements. On the basis of compilation analysis we suggest a consensus sequence for plant H3 histones which differs from that of animal's by four amino acid changes.

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References

    1. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 - PubMed
    1. Gene. 1988 Nov 15;71(1):217-23 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
    1. Nucleic Acids Res. 1980 Dec 11;8(23):5725-37 - PubMed
    1. Cell. 1981 Aug;25(2):301-13 - PubMed