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. 2012 Jul;2(3):227-34.
doi: 10.1016/s2225-4110(16)30104-3.

Stachyose: One of the Active Fibroblast-proliferating Components in the Root of Rehmanniae Radix ( dì huáng)

Patrick Kwok-Kin Lai  1 , Ming-Ho To  1 , Kit-Man Lau  1 , Cheuk-Lun Liu  1 , Ling Cheng  1 , Kwok-Pui Fung  2 , Ping-Chung Leung  1 , Clara Bik-San Lau  1
Affiliations

Stachyose: One of the Active Fibroblast-proliferating Components in the Root of Rehmanniae Radix ( dì huáng)

Patrick Kwok-Kin Lai et al. J Tradit Complement Med. 2012 Jul.

Abstract

This study aimed to investigate and compare the fibroblast-proliferating activities of different Rehmanniae Radix (RR) samples and its chemical components using human normal fibroblast cells Hs27. Those active components were quantified in differently treated RR samples using UPLC so as to correlate activity with component content. Our results showed that dried RR aqueous extract exhibited the most potent fibroblast-proliferating activity. Stronger effect was observed when ethanol with heating was applied in the extraction process. Stachyose and verbascoside were demonstrated for their first time to exhibit significant stimulatory effects on fibroblast proliferation. However, the proliferating effect of dried RR extract did not correlate with the stachyose content, and verbascoside was not responsible for the fibroblast proliferative effect of RR since it was undetectable in all samples. In conclusion, stachyose only contributed in part to the activity of RR, suggesting that other active components might be present and yet to be found.

Keywords: Fibroblast proliferation; Rehmannia glutinosa; Rehmanniae Radix; Stachyose; Verbascoside.

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Figures

Figure 1
Figure 1
Chemical structures of five known chemical components in RR. A: catalpol; B: 5-hydroxymethyl-2-furaldehyde; C: manninotriose; D: stachyose; E: verbascoside.
Figure 2
Figure 2
Fibroblast proliferation effect of the aqueous crude extracts of three different forms of RR. * denotes p<0.05 when compared with 100% control and # denotes p<0.05 when compared with dried RR using Student's t-test.
Figure 3
Figure 3
Effect of dried RR crude extracts prepared from different extraction methods on fibroblast proliferation. Data are mean±SD (n=3). * denotes p<0.05 and ** denotes p<0.01 when compared with 100% control using Student's t-test.
Figure 4
Figure 4
Effect of five chemical components on fibroblast proliferation. A: Stachyose; B: Manninotriose; C: Catalpol; D: 5-HMF; E: Verbascoside. Data are mean±SD (n≥3). *denotes p<0.05 and ** denotes p<0.01 when compared with 100% control using Student's t-test.
Figure S1
Figure S1
UPLC profiles of stachyose present in four dried RR crude extracts
Figure S2
Figure S2
UPLC profiles for the detection of verbascoside in four dried RR crude extracts
See this image and copyright information in PMC

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