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. 2014 Mar 15;10(4):396-403.
doi: 10.7150/ijbs.8193. eCollection 2014.

SRC-1 and Twist1 expression positively correlates with a poor prognosis in human breast cancer

Yan Xu  1 , Baoquan Hu  1 , Li Qin  2 , Lianhua Zhao  3 , Qiang Wang  4 , Qingliang Wang  4 , Yixiang Xu  2 , Jun Jiang  1
Affiliations

SRC-1 and Twist1 expression positively correlates with a poor prognosis in human breast cancer

Yan Xu et al. Int J Biol Sci. .

Abstract

To evaluate the possible prognostic value of Steroid Receptor Coactivator-1 (SRC-1) and Twist1 expression in human breast cancer, we examined SRC-1 and Twist1 expression using immunohistochemistry on tissue microarray sections containing 137 breast cancer specimens. All patients were followed up for a median of 5 years following surgery. Survival curves were generated using the Kaplan-Meier method. Multivariate analysis was performed using the Cox proportional hazard regression model to assess the prognostic values. The results showed a positive correlation between SRC-1 and Twist1 expression at protein levels (P < 0.001). Also, SRC-1 expression positively correlated with HER2 expression (P = 0.024). The protein expression of Twist1 positively associated with lymph node metastasis (P < 0.001), but inversely correlated with PR status (P = 0.041). Patients with SRC-1 or Twist1-positive expression exhibited poorer overall survival (OS) and disease-free survival (DFS) than did those with SRC-1 or Twist1-negative expression (P < 0.05 for all). In addition, SRC-1-negativeive/Twist1-negative patients had the best OS and DFS (P < 0.01 for both). In multivariate survival analysis, SRC-1 expression, tumor stage, and PR were found to be independent prognostic factors related to OS (P = 0.019, < 0.001 and 0.02, respectively) and Twist1 expression, lymph node status and PR were independent predictors of DFS (P = 0.006, 0.001 and 0.029, respectively). These results suggest that a combined SRC-1/Twist1 expression status could improve the prognostic judgment for breast cancer patients.

Keywords: Breast cancer; Correlation; Prognosis.; SRC-1; Twist1.

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Conflict of interest statement

Conflicts of Interest: The authors declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1
Representative immunohistochemical staining of SRC-1(A-D) and Twist1 (E-H) in human breast cancer. (A, E) Negative staining, score 0. (B,F)Weak positive staining, (B) Score 6 (intensity 1, percentage 5), (F) Score 4 (intensity 1, percentage 3); (C, G) Moderate positive staining, (C) Score 7 (intensity 2, percentage 5),(G) Score 7 (intensity 2, percentage 5); ( D, H) Strong positive staining.(D) Score 8 (intensity 3, percentage 5), (H) Score 8 (intensity 3, percentage 5). Original magnification, ×400.
Figure 2
Figure 2
Correlation between SRC-1 and Twist1 expression in breast cancer samples. IHC against SRC-1 and Twist1 was performed on TMA sections' containing human breast cancer specimens. The typical staining images for high and low expression of SRC-1 and Twist1 were shown. Statistical analysis of the expression patterns revealed that there was a positive correlation between SRC-1 and Twist1 expression (r = 0.321, P < 0.001).
Figure 3
Figure 3
Kaplan-Meier overall survival (OS) and disease-free survival (DFS) analysis of breast cancer patients.
See this image and copyright information in PMC

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