Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Apr;8(4):1213-6.
doi: 10.1002/j.1460-2075.1989.tb03494.x.

Mutations in 16S ribosomal RNA disrupt antibiotic--RNA interactions

E A De Stasio  1 , D Moazed, H F Noller, A E Dahlberg
Affiliations

Mutations in 16S ribosomal RNA disrupt antibiotic--RNA interactions

E A De Stasio et al. EMBO J. 1989 Apr.

Abstract

Two of six mutations at a base-paired site in Escherichia coli 16S rRNA confer resistance to nine different aminoglycoside antibiotics in vivo. Chemical probing of mutant and wild-type ribosomes in the presence of paromomycin indicates that interactions between the antibiotic and 16S rRNA in mutant ribosomes are disrupted. The altered interactions measured in vitro correlate precisely with resistance seen in vivo and may be attributable to specific structural changes observed in the mutant rRNA.

PubMed Disclaimer

References

    1. Science. 1965 Sep 3;149(3688):1096-8 - PubMed
    1. Nature. 1969 Apr 26;222(5191):333-9 - PubMed
    1. Science. 1969 Dec 5;166(3910):1282-4 - PubMed
    1. Proc Natl Acad Sci U S A. 1974 Oct;71(10):3814-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1760-4 - PubMed

Publication types

LinkOut - more resources