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. 1989 Apr;80(4):295-8.
doi: 10.1111/j.1349-7006.1989.tb02308.x.

Demethylation by 5-azacytidine results in the expression of hepatitis B virus surface antigen in transgenic mice

K Araki  1 , J Miyazaki, T Tsurimoto, T Inomoto, T Iwanaga, K Matsubara, K Yamamura
Affiliations

Demethylation by 5-azacytidine results in the expression of hepatitis B virus surface antigen in transgenic mice

K Araki et al. Jpn J Cancer Res. 1989 Apr.

Abstract

In 14p3HB transgenic mice, which carry three tandem copies of hepatitis B virus (HBV) DNA, the HBV DNA was significantly methylated and no viral proteins were produced. To analyze the causal relationship between hypermethylation and gene inactivity, 5-azacytidine was injected into the mice to demethylate HBV DNA. When postnatal 14p3HB mice were treated with the drug, hepatitis virus surface antigen was produced in these mice by 3 weeks of age, and the integrated HBV DNA of the liver was less heavily methylated. Our results suggest that injection of 5-azacytidine can be used to efficiently activate a silent transgene such as HBV DNA in transgenic mice.

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