Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Apr 14:13:14.
doi: 10.1186/1476-0711-13-14.

Antimicrobial susceptibility pattern of bacterial isolates from wound infection and their sensitivity to alternative topical agents at Jimma University Specialized Hospital, South-West Ethiopia

Mohammedaman Mama, Alemseged Abdissa, Tsegaye Sewunet  1
Affiliations

Antimicrobial susceptibility pattern of bacterial isolates from wound infection and their sensitivity to alternative topical agents at Jimma University Specialized Hospital, South-West Ethiopia

Mohammedaman Mama et al. Ann Clin Microbiol Antimicrob. .

Abstract

Background: Wound infection is one of the health problems that are caused and aggravated by the invasion of pathogenic organisms. Information on local pathogens and sensitivity to antimicrobial agents, and topical agents like acetic acid is crucial for successful treatment of wounds.

Objectives: To determine antimicrobial susceptibility pattern of bacterial isolates from wound infection and their sensitivity to alternative topical agents at Jimma University Specialized Hospital.

Methods: A cross sectional study was conducted among patients with wound infection visiting Jimma University Specialized Hospital, from May to September 2013. Wound swab was collected using sterile cotton swabs and processed for bacterial isolation and susceptibility testing to antimicrobial agents, acetic acid, hydrogen peroxide and dabkin solution following standard bacteriological techniques. Biochemical tests were done to identify the species of the organisms. Sensitivity testing was done using Kirby- Baur disk diffusion method. Minimum inhibitory and bactericidal concentration was done using tube dilution method.

Results: In this study 145 bacterial isolates were recovered from 150 specimens showing an isolation rate of 87.3%. The predominant bacteria isolated from the infected wounds were Staphylococcus aureus 47 (32.4%) followed by Escherichia coli 29 (20%), Proteus species 23 (16%), Coagulase negative Staphylococci 21 (14.5%), Klebsiella pneumoniae 14 (10%) and Pseudomonas aeruginosa 11 (8%). All isolates showed high frequency of resistance to ampicillin, penicillin, cephalothin and tetracycline. The overall multiple drug resistance patterns were found to be 85%. Acetic acid (0.5%), Dabkin solution (1%) and 3% hydrogen peroxide were bactericidal to all isolated bacteria and lethal effect observed when applied for 10 minutes.

Conclusions: On in vitro sensitivity testing, ampicillin, penicillin, cephalothin and tetracycline were the least effective. Gentamicin, norfloxacin, ciprofloxacin, vancomycin and amikacin were the most effective antibiotics. Acetic acid (0.5%), dabkin solution (1%) and H2O2 (3%) were bactericidal to all isolates.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Percentage of bacteria isolated from patients with infected wounds at JUSH, Jimma, May-September, 2013.
See this image and copyright information in PMC

References

    1. Ndip RN, Takang AEM, Echakachi CM, Malongue A, Akoachere J-FTK. In vitro antimicrobial activity of selected honeys on clinical isolates of Helicobacter pylori. Afr Health Sci. 2007;13(4):228–231. - PMC - PubMed
    1. Dai T, Huang Y-Y, Sharma SK, Hashmi JT, Kurup DB, Hamblin MR. Topical antimicrobials for burn wound infections. Recent Pat Antiinfect Drug Discov. 2010;13(2):124–151. doi: 10.2174/157489110791233522. - DOI - PMC - PubMed
    1. Kotz P, Fisher J, McCluskey P, Hartwell SD, Dharma H. Use of a new silver barrier dressing, ALLEVYN Ag in exuding chronic wounds. Int Wound J. 2009;13:186–194. doi: 10.1111/j.1742-481X.2009.00608.x. - DOI - PMC - PubMed
    1. Taiwo S, Okesina A, Onile B. In vitro antimicrobial susceptibility pattern of bacterial isolates from wound infections in University of Ilorin Teaching Hospital. Afr J Clin Exp Microbiol. 2002;13(1):6–10.
    1. Al-Waili NS, Salom K, Al-Ghamdi AA. Honey for wound healing, ulcers, and burns. TheScientificWorldJOURNAL. 2011;13:766–787. - PMC - PubMed

Publication types

MeSH terms

Substances