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Review
. 2014 Apr 11;6(4):1672-700.
doi: 10.3390/v6041672.

Matrix and backstage: cellular substrates for viral vaccines

Ingo Jordan  1 , Volker Sandig  2
Affiliations
Review

Matrix and backstage: cellular substrates for viral vaccines

Ingo Jordan et al. Viruses. .

Abstract

Vaccines are complex products that are manufactured in highly dynamic processes. Cellular substrates are one critical component that can have an enormous impact on reactogenicity of the final preparation, level of attenuation of a live virus, yield of infectious units or antigens, and cost per vaccine dose. Such parameters contribute to feasibility and affordability of vaccine programs both in industrialized countries and developing regions. This review summarizes the diversity of cellular substrates for propagation of viral vaccines from primary tissue explants and embryonated chicken eggs to designed continuous cell lines of human and avian origin.

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Figures

Figure 1
Figure 1
Time line of cell line derivations (left to the time bar) and key developments (right side) referred to in the text. YFV 17D, yellow fever virus 17D strain; CEF, chicken embryo fibroblasts; AFEB, American Forces Epidemiological Board; PMKC, primary monkey kidney cell; IPV and OPV, inactivated and oral polio vaccine, respectively; HAdV, human adenovirus; MVA, modified vaccinia virus Ankara.
See this image and copyright information in PMC

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