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Review
. 2014 Apr 14:106:28.5.1-28.5.12.
doi: 10.1002/0471142727.mb2805s106.

Eradication of Mycoplasma contaminations from cell cultures

Cord C Uphoff  1 , Hans G Drexler  1
Affiliations
Review

Eradication of Mycoplasma contaminations from cell cultures

Cord C Uphoff et al. Curr Protoc Mol Biol. .

Abstract

Mycoplasma contaminations have a multitude of effects on cultured cell lines that may influence the results of experiments or pollute bioactive substances isolated from the eukaryotic cells. The elimination of mycoplasma contaminations from cell cultures with antibiotics has been proven to be a practical alternative to discarding and re-establishing important or irreplaceable cell lines. Different fluoroquinolones, tetracyclins, pleuromutilins, and macrolides shown to have strong anti-mycoplasma properties are employed for the decontamination. These antibiotics are applied as single treatments, as combination treatment of two antibiotics in parallel or successively, or in combination with a surface-active peptide to enhance the action of the antibiotic. The protocols in this unit allow eradication of mycoplasmas, prevention of the development of resistant mycoplasma strains, and potential cure of heavily contaminated and damaged cells. Consistent and permanent alterations to eukaryotic cells attributable to the treatment have not been demonstrated.

Keywords: antibiotic treatment; contamination; elimination; mycoplasma.

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References

Literature Cited

    1. Armstrong, S.E. , Mariano, J.A. , and Lundin, D.J. 2010. The scope of mycoplasma contamination within the biopharmaceutical industry. Biologicals 38:211-213.
    1. Drexler, H.G. and Uphoff, C.C. 2002. Mycoplasma contamination of cell cultures: Incidence, sources, effects, detection, elimination, prevention. Cytotechnology 39:75-90.
    1. Nir-Paz, R. , Prévost, M.-C. , Nicolas, P. , Blanchard, A. , and Wróblewski, H. 2002. Susceptibilities of Mycoplasma fermentans and Mycoplasma hyorhinis to membrane-active peptides and enrofloxacin in human tissue cell cultures. Antimicrob. Agents Chemother. 46:1218-1225.
    1. Rottem, S. and Barile, M.F. 1993. Beware of mycoplasmas. Tibtech 11:143-151.
    1. Schmidt, J. and Erfle, V. 1984. Elimination of mycoplasma from cell cultures and establishment of mycoplasma-free cell lines. Exp. Cell Res. 152:565-570.

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