Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014:2014:979584.
doi: 10.1155/2014/979584. Epub 2014 Mar 9.

Escherichia coli Strains Isolated from the Uteri Horn, Mouth, and Rectum of Bitches Suffering from Pyometra: Virulence Factors, Antimicrobial Susceptibilities, and Clonal Relationships among Strains

Juliana M A Agostinho  1 , Andressa de Souza  2 , Ruben P Schocken-Iturrino  3 , Lívia G Beraldo  1 , Clarissa A Borges  1 , Fernando A Avila  3 , José M Marin  4
Affiliations

Escherichia coli Strains Isolated from the Uteri Horn, Mouth, and Rectum of Bitches Suffering from Pyometra: Virulence Factors, Antimicrobial Susceptibilities, and Clonal Relationships among Strains

Juliana M A Agostinho et al. Int J Microbiol. 2014.

Abstract

Pyometra is recognized as one of the main causes of disease and death in the bitch, and Escherichia coli is the major pathogen associated with this disease. In this study, 70 E. coli isolates from the uteri horn, mouth, and rectum of bitches suffering from the disease and 43 E. coli isolates from the rectum of clinically healthy bitches were examined for the presence of uropathogenic virulence genes and susceptibility to antimicrobial drugs. DNA profiles of isolates from uteri horn and mouth in bitches with pyometra were compared by REP, ERIC, and BOX-PCR. Virulence gene frequencies detected in isolates from canine pyometra were as follows: 95.7% fim, 27.1% iss, 25.7% hly, 18.5% iuc, and 17.1% usp. Predominant resistance was determined for cephalothin, ampicillin, and nalidixic acid among the isolates from all sites examined. Multidrug resistance was found on ∼ 50% pyometra isolates. Using the genotypic methods some isolates from uteri, pus, and saliva of the same bitch proved to have identical DNA profiles which is a reason for concern due to the close relationship between household pets and humans.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Dendrogram constructed by UPGMA method using genetic distances obtained by REP-PCR analysis of E. coli isolates from bitches suffering pyometra: 2/18-bitch 2 isolate 18 from uteri pus; 2/36-bitch 2 isolate 36 from saliva; 2/41-bitch 2 isolate 41 from saliva; 3/51-bitch 3 isolate 51 from saliva; 3/59-bitch 3 isolate 59 from uteri pus; 4/65-bitch 4 isolate 65 from saliva; 4/71-bitch 4 isolate 71 from uteri pus.
See this image and copyright information in PMC

References

    1. Grooters AM. Diseases of the ovaries and uterus. In: Birchards SJ, Sherding RG, editors. Saunders Manual of Small Animal Practice. W.B. Saunders; 1994. p. p. 1467.
    1. Fransson BA, Ragle CA. Canine pyometra: an update on pathogenesis and treatment. Compendium on Continuing Education for the Practicing Veterinarian. 2003;25(8):602–612.
    1. Smith FO. Canine pyometra. Theriogenology. 2006;66(3):610–612. - PubMed
    1. Hagman R, Kühn I. Escherichia coli strains isolated from the uterus and urinary bladder of bitches suffering from pyometra: comparison by restriction enzyme digestion and pulsed-field gel electrophoresis. Veterinary Microbiology. 2002;84(1-2):143–153. - PubMed
    1. Sandholm M, Vasenius H, Kivisto AK. Pathogenesis of canine pyometra. Journal of American Veterinary Medical Association. 1975;167:1006–1010. - PubMed

LinkOut - more resources