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. 1989 May-Jun;61(3):107-11.

[Isolation and partial characterization of poly-(a)+mRNA of soybean grown in the presence of gibberellic acid]

[Article in Russian]
D I Dzhokhadze, N A Mamulashvili, N V Lomidze, L A Nazarova
  • PMID: 2473562

[Isolation and partial characterization of poly-(a)+mRNA of soybean grown in the presence of gibberellic acid]

[Article in Russian]
D I Dzhokhadze et al. Ukr Biokhim Zh (1978). 1989 May-Jun.

Abstract

Poly(A)+mRNA was prepared by chromatography on the oligo (dT)-cellulose column from total RNA of epicotyls of soybean grown on the medium with gibberellic acid. Poly(A)+mRNA was prepared by the same method from control plants. Total RNA, poly-A and poly(A)+mRNAs were analyzed by electrophoresis on 1.5% agarose gel under denaturing conditions. The poly(A)+mRNA distribution was shown to be of polydispersional character, cDNA was synthesized by reverse transcription. Basing on cDNA-poly(A)+mRNA hybridization a conclusion is drawn that gibberellic acid may activate new types of mRNAs and, probably, increases the synthesis of some poly(A)+mRNA populations.

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