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. 2014 May 30;289(22):15141-53.
doi: 10.1074/jbc.X114.574376. Epub 2014 Apr 15.

Life in a sea of oxygen

John D Lipscomb  1
Affiliations

Life in a sea of oxygen

John D Lipscomb. J Biol Chem. .
No abstract available

Keywords: Cytochrome P450; Dioxygenase; Heme; Metalloenzymes; Spectroscopy.

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Figures

FIGURE 1.
FIGURE 1.
Oxygenase friends and pioneers. Left, I. C. “Gunny” Gunsalus in 1976. Center, Lawrence Que, Jr., in 2013. Right, Eckard Münck in 1977.
FIGURE 2.
FIGURE 2.
Early contributors to P450cam research celebrating Stephen Sligar's 60th birthday. Left to right: Thomas Poulos (University of California, Irvine), John Lipscomb, Stephen Sligar (University of Illinois), and John Dawson (University of South Carolina). The photo was provided by Masao Ikeda-Saito (Tohoku University, Miyagi, Japan).
FIGURE 3.
FIGURE 3.
Crystals of 3,4-PCD. These crystals led to the first structure of a non-heme iron enzyme.
FIGURE 4.
FIGURE 4.
Extradiol reaction mechanism proposed in 1986. Most elements of this mechanism have been borne out by subsequent work, except that the Fe(II)-superoxo attack on the ring is likely to be at the hydroxyl-bearing carbon para to the carboxyl substituent. This figure was reproduced from Scheme 2 in Ref. .
FIGURE 5.
FIGURE 5.
Setting up for a group picture at the 2nd International Symposium on Oxygenases and Oxygen Metabolism in Hokone, Japan, in 1981. Left to right: Bo Malmström, Jack Peisach, Minor J. Coon, Konrad Bloch, Osamu Hayaishi, Howard Mason, I. C. Gunsalus, Lars Ernster.
FIGURE 6.
FIGURE 6.
Extradiol dioxygenase intermediates trapped in three different active sites in one crystal (44). The structure is from Protein Data Bank ID 2IGA.
FIGURE 7.
FIGURE 7.
Intermediates in the MMO catalytic cycle proposed in 1993. The figure is reproduced from Scheme 2 of Ref. . The inset in the center of the cycle is the structure for compound Q proposed in 1997 in Ref. .
See this image and copyright information in PMC

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References

    1. Tyson C. A., Lipscomb J. D., Gunsalus I. C. (1972) The role of putidaredoxin and P450cam in methylene hydroxylation. J. Biol. Chem. 247, 5777–5784 - PubMed
    1. Gunsalus I. C., Tyson C. A., Lipscomb J. D. (1973) Cytochrome P-450 reduction and oxygenation systems. in Oxidases and Related Redox Systems (King T. E., Mason H. S., Morrison M., eds) pp 583–603, University Park Press, Baltimore
    1. Sharrock M., Debrunner P. G., Schulz C., Lipscomb J. D., Marshall V., Gunsalus I. C. (1976) Cytochrome P450cam and its complexes. Mössbauer parameters of the heme iron. Biochim. Biophys. Acta 420, 8–26 - PubMed
    1. Sharrock M., Münck E., Debrunner P. G., Marshall V., Lipscomb J. D., Gunsalus I. C. (1973) Mössbauer studies of cytochrome P-450cam. Biochemistry 12, 258–265 - PubMed
    1. Gunsalus I. C., Meeks J. R., Lipscomb J. D., Marshall V. P., Münck E., Debrunner P. G. (1974) Bacterial monooxygenases–the P450 cytochrome system. in Molecular Mechanisms of Oxygen Activation (Hayaishi O., ed) pp. 561–613, Academic Press, New York

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