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. 1989 Aug;37(8):1299-301.
doi: 10.1177/37.8.2474024.

Indoxyl-tetranitro blue tetrazolium method for detection of alkaline phosphatase in immunohistochemistry

M Brenan  1 , M L Bath
Affiliations

Indoxyl-tetranitro blue tetrazolium method for detection of alkaline phosphatase in immunohistochemistry

M Brenan et al. J Histochem Cytochem. 1989 Aug.

Abstract

A sensitive method for detection of alkaline phosphatase in immunohistochemistry, using lymphoid cells, has been optimized. The conditions for staining are 0.23 mM 5-bromo-4-chloro-indoxyl phosphate, 0.55 mM tetranitro blue tetrazolium, 2.0 mM levamisole, 5.0 mM sodium azide, 10.0 mM magnesium chloride, and 0.15 mM 1-methoxyphenazine methosulfate dissolved in 100 mM Tris-HCl buffer, pH 9.5.

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