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Comment
. 2014 Aug;3(4):1038-40.
doi: 10.1002/cam4.243. Epub 2014 Apr 17.

Regarding human cytomegalovirus in neuroblastoma

Ola Forslund  1 , Linda Holmquist Mengelbier, David Gisselsson
Affiliations
Comment

Regarding human cytomegalovirus in neuroblastoma

Ola Forslund et al. Cancer Med. 2014 Aug.

Abstract

Wolmer-Solberg et al., reported that six human neuroblastoma cell lines and the vast majority of clinical neuroblastoma samples contained HCMV DNA and expressed HCMV proteins. We could not replicate the data and therefore remain skeptical towards the prevalence of HCMV DNA in neuroblastomas.

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Figures

Figure 1
Figure 1
Limit of detection for HCMV was 10 copies/real-time PCR for each target gene IE(Ct: 40.9), pp150 (Ct: 37.9) and UL55/gB (Ct: 40.6). Cycle threshold (Ct) was automatically calculated by the ABI 7500 software v2.0.6. Input of 100, 10 and 1 HCMV copies are shown.
Figure 2
Figure 2
Neuroblastoma cell lines in triplicate (SH-SY5Y, SK-N-BE(2)C, SK-N-AS and IMR-32) were all negative for HCMV-DNA. Positive controls of 1000 HCMV-copies/real-time PCR generated expected curves for each target gene IE (Ct: 33.9), pp150 (Ct: 32.0) and UL55/gB (Ct: 32.9). Cycle threshold (Ct) was automatically calculated by the ABI 7500 software v2.0.6.
See this image and copyright information in PMC

Comment on

References

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