Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Mar;6(3):158-9.
doi: 10.4103/1947-2714.128481.

Western blot: protein transfer overview

Affiliations

Western blot: protein transfer overview

Janay Gibbons. N Am J Med Sci. 2014 Mar.
No abstract available

PubMed Disclaimer

Figures

Figure 1
Figure 1
A submersed electrophorator. Figure 7 from ‘Western Blot: Technique, Theory, and Trouble Shooting’.[1]
Figure 2
Figure 2
A western blot of collagen-induced platelet activation over time using semi-dry protein transfer

References

    1. Mahmood T, Yang PC. Western Blot: Technique, theory, and trouble shooting. N Am J Med Sci. 2012;4:429–34. - PMC - PubMed
    1. Kurien BT, Scofield RH. Western blotting. Methods. 2006;38:283–93. - PubMed
    1. MacPhee DJ. Methodological considerations for improving Western blot analysis. J Pharmacol Toxicol Methods. 2010;61:171–7. - PubMed
    1. Kurien BT, Scofield RH. Protein blotting: A review. J Immunol Methods. 2003;274:1–15. - PubMed
    1. Kurien BT, Scofield RH. Multiple immunoblots after non-electrophoretic bidirectional transfer of a single SDS–PAGE gel with multiple antigens. J Immunol Methods. 1997;205:91–4. - PubMed