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. 2014 Apr 17:7:244.
doi: 10.1186/1756-0500-7-244.

Antifungal and antioxidant activity of Crassocephalum bauchiense (Hutch.) Milne-Redh ethyl acetate extract and fractions (Asteraceae)

Raymond Simplice Mouokeu  1 , Rosalie A Ngono Ngane, Guy S S Njateng, Monique O Kamtchueng, Jules-Roger Kuiate
Affiliations

Antifungal and antioxidant activity of Crassocephalum bauchiense (Hutch.) Milne-Redh ethyl acetate extract and fractions (Asteraceae)

Raymond Simplice Mouokeu et al. BMC Res Notes. .

Abstract

Background: Crassocephalum bauchiense is a flowering plant, found in the West Region of Cameroon. Previous studied has highlighted the antibacterial and the dermal toxicological safety as well as the immunomodulatory activities of the ethyl acetate extract of its dry leaves. As an extension of the previous researches, the current work has been undertaken to evaluate the in vitro antifungal and antioxidant activities of C. bauchiense dried leaves ethyl acetate extract and fractions.

Methods: The extract was obtained by maceration in ethyl acetate and further fractionated into six fractions labeled F1 to F6 by flash chromatography. The antifungal activity of the extract and fractions against yeasts and dermatophytes was evaluated using broth microdilution method. Antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO) and β-carotene - linoleic acid assays.

Results: The extract (MIC = 0.125 - 4 mg/ml) was found to be more active on dermatophytes and yeasts compared to the fractions. The ethyl acetate extract and fractions exhibited strong scavenging activity on DPPH (CI50 = 28.57 - 389.38 μg/ml). The fractions F3 and F6 expressed best antioxidant activity on DPPH radicals compared to the crude extract.

Conclusion: The results of these findings clearly showed that C. bauchiense ethyl acetate extract has a significant antifungal and antioxidant activity. It is therefore a source of active compounds that might be used as antifungal and antioxidant agents.

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References

    1. Mouokeu RS, Ngono Ngane AR, Lunga PK, Koanga MM, Tiabou AT, Njateng GSS, Tamokou JDD, Kuiate JR. Antibacterial and dermal toxicological profiles of ethyl acetate extract from Crassocephalum bauchiense (Hutch.) Milne-Redh (Asteraceae) BMC Complement Altern Med. 2011;11:43. doi: 10.1186/1472-6882-11-43. - DOI - PMC - PubMed
    1. Mouokeu RS, Ngono Ngane RA, Tume C, Kamtchueng MO, Njateng GSS, Dzoyem JP, Tamokou JD, Kuiate JR. Immunomodulatory activity of ethyl acetate extract and fractions from leaves of Crassocephalum bauchiense (Asteraceae) Pharmacol. 2013;4(1):38–47.
    1. Yehouenou B, Wotto V, Bankole H, Sessou P, Noudogbessi JP, Sohounhloue D. Chemical study and antimicrobial activities of volatile extracts from fresh leaves of Crassocephalum rubens (juss & jack) s. moore against food-borne pathogens. St Cerc St CICBIA. 2010;11(3):pp. 341–pp. 349.
    1. Tabopda TK, Fotso GW, Ngoupayo J, Mitaine-Offer ACL, Ngadjui BT, Lacaille-Dubois MA. Antimicrobial Dihydroisocoumarins from Crassocephalum biafrae. Planta Med. 2009;75(11):1258–1261. doi: 10.1055/s-0029-1185545. - DOI - PubMed
    1. Mukazayire MJ, Tomani JC, Okusa NP, Chalchat JC, Stevigny C, Duez P. Chemical composition and evaluation of antioxidant and antimicrobial properties of Ocimum lamiifolium and Crassocephalum vitellinum essential oils. Planta Med. 2008;74:302. doi: 10.1055/s-2008-1034305. - DOI - PubMed

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