Combined detection tumor markers for diagnosis and prognosis of gallbladder cancer

Abstract

Aim: To clarify the value of combined use of markers for the diagnosis of gallbladder cancer and prediction of its prognosis.

Methods: Serum cancer antigens (CA)199, CA242, carcinoembryonic antigen (CEA), and CA125 levels were measured in 78 patients with gallbladder cancer (GBC), 78 patients with benign gallbladder diseases, and 78 healthy controls using electrochemiluminescence. CA199, CA242, CEA, and CA125 levels and positive rates were analyzed and evaluated pre- and post-operatively. Receiver operator characteristic curves were used to determine diagnostic sensitivity and specificity of GBC. Survival time analysis, including survival curves, and multivariate survival analysis of a Cox proportional hazards model was performed to evaluate independent prognostic factors.

Results: Serum CA242, CA125, and CA199 levels in the GBC group were significantly higher when compared with those in the benign gallbladder disease and healthy control groups (P < 0.01). With a single tumor marker for GBC diagnosis, the sensitivity of CA199 was the highest (71.7%), with the highest specificity being in CA242 (98.7%). Diagnostic accuracy was highest with a combination of CA199, CA242, and CA125 (69.2%). CA242 could be regarded as a tumor marker of GBC infiltration in the early stage. The sensitivity of CA199 and CA242 increased with progression of GBC and advanced lymph node metastasis (P < 0.05). The 78 GBC patients were followed up for 6-12 mo (mean: 8 mo), during which time serum CA199, CA125, and CA242 levels in the recurrence group were significantly higher than in patients without recurrence (P < 0.01). The post-operative serum CA199, CA125, and CA242 levels in the non-recurrence group were significantly lower than those in the GBC group (P < 0.01). Multivariate survival analysis using a Cox proportional hazards model showed that cancer of the gallbladder neck and CA199 expression level were independent prognostic factors.

Conclusion: CA242 is a marker of GBC infiltration in the early stage. CA199 and cancer of the gallbladder neck are therapeutic and prognostic markers.

Keywords: Combined detection; Diagnosis; Gallbladder cancer; Prognosis; Tumor marker.

Figure 2

Analysis of overall survival curves. Overall survival curves for 78 patients with gallbladder cancer (GBC). Kaplan-Meier survival curves in GBC patients with a Cox proportional hazards model for multivariate regression analysis.

Figure 1

Receiver operating characteristic curve. Receiver operating characteristic curves showing diagnostic performance of cancer antigens (CA) CA199, CA125, CA242, and carcinoembryonic antigen (CEA). The sensitivity of CA199 was the highest (71.7%) and the specificity of CA242 was the highest (98.7%).