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. 1988 Sep;134(9):2599-604.
doi: 10.1099/00221287-134-9-2599.

Production and characterization of monoclonal antibodies specific for Mycobacterium bovis

P R Wood  1 , J Ripper, A J Radford, P G Bundesen, D B Rylatt, L E Cottis, M John, P Plackett
Affiliations

Production and characterization of monoclonal antibodies specific for Mycobacterium bovis

P R Wood et al. J Gen Microbiol. 1988 Sep.

Abstract

A series of monoclonal antibodies (MAbs), specific for Mycobacterium bovis and BCG strains, were tested extensively for cross-reactivity to a wide range of mycobacterial species using ELISA, Western blotting and dot-blot analysis. The MAbs bound specifically to M. bovis and BCG and showed limited cross-reactivity with some strains of M. tuberculosis. All these MAbs recognized a 22 kDa protein previously termed MPB70, and by competitive ELISA analysis appeared to detect at least three M. bovis-specific determinants on the MPB70 molecule.

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