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. 2014 Apr 7;14(4):e13100.
doi: 10.5812/hepatmon.13100. eCollection 2014 Apr.

Lack of Association Between Transforming Growth Factor Beta 1 -509C/T and +915G/C Polymorphisms and Chronic Hepatitis B in Iranian Patients

Armin Hosseini Razavi  1 , Pedram Azimzadeh  2 , Seyed Reza Mohebbi  2 , Seyed Masoud Hosseini  3 , Sara Romani  2 , Mahsa Khanyaghma  2 , Yasin Hatami  4 , Afsaneh Sharifian  2 , Mohammad Reza Zali  2
Affiliations

Lack of Association Between Transforming Growth Factor Beta 1 -509C/T and +915G/C Polymorphisms and Chronic Hepatitis B in Iranian Patients

Armin Hosseini Razavi et al. Hepat Mon. .

Erratum in

  • Hepat Mon. 2014 Aug;14(8):e20548

Abstract

Background: Chronic hepatitis B is one of the world's major health concerns [corrected]. The etiological agent of this infection is hepatitis B virus (HBV), which can evade the immune system response. Transforming growth factor beta 1 (TGF-β1) can act against HBV by suppressing the viral replication. The TGF-β1 also plays an important role in preventing liver damage in chronically HBV infected patients.

Objectives: In this study, the association of TGF-β1 +915G/C and -509C/T gene polymorphisms with chronic hepatitis B was evaluated in Iranian patients.

Materials and methods: A population-based case-control study was conducted in Taleghani Hospital, Tehran. A number of 220 patients with chronic hepatitis B and the same number of healthy control subjects were designated the case and the control groups. The PCR-Restriction Fragment Length Polymorphism Method (PCR-RFLP) method was used for genotyping both polymorphisms. Ten percent of the control samples were sequenced to confirm the results.

Results: No statically significant differences in genotype distribution and allele frequency were observed for both polymorphisms between healthy controls and patients with chronic hepatitis B.

Conclusions: There was no association between TGF-β1 -509C/T and +915G/C polymorphisms with chronic hepatitis B and it seems that these changes do not play a significant role in increasing the risk of chronic infection in Iranian patients [corrected].

Keywords: Hepatitis B, Chronic; Polymorphism, Genetic; Transforming Growth Factor beta 1.

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Figures

Figure 1.
Figure 1.. Products of Enzymatic Digestion and Their Fragments Size on Agarose Gel Electrophoresis
A) Eco81I fragments (-509C/T polymorphism), B) BglI fragments (+915G/C polymorphism), F1) CC genotype of -509C/T and GC genotype of +915G/C, F2) TT genotype of -509C/T and GG genotype of +915G/C, F3) CT genotype of -509C/T and CC genotype of +915G/C, F4) Positive control, F5) Negative control (PCR product), L1) 100 bp DNA ladder, L2) 50 bp DNA ladder.
Figure 2.
Figure 2.. Sequence of Heterozygous +915T/C
Arrow shows G and C alleles in this polymorphism
See this image and copyright information in PMC

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