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. 2014 Apr;27(2):169-74.
doi: 10.15274/NRJ-2014-10028. Epub 2014 Apr 18.

Neural stem cells and glioblastoma

Rossella Rispoli  1 , Carlo Conti  2 , Paolo Celli  3 , Emanuela Caroli  3 , Sandro Carletti  2
Affiliations

Neural stem cells and glioblastoma

Rossella Rispoli et al. Neuroradiol J. 2014 Apr.

Abstract

Glioblastoma multiforme represents one of the most common brain cancers with a rather heterogeneous cellular composition, as indicated by the term "multiforme". Recent reports have described the isolation and identification of cancer neural stem cells from human adult glioblastoma multiforme, which possess the capacity to establish, sustain, and expand these tumours, even under the challenging settings posed by serial transplantation experiments. Our study focused on the distribution of neural cancer stem cells inside the tumour. The study is divided into three phases: removal of tumoral specimens in different areas of the tumour (centre, periphery, marginal zone) in an operative room equipped with a 1.5 T scanner; isolation and characterization of neural cancer stem cells from human adult glioblastoma multiforme; identification of neural cancer stem cell distribution inside the tumour.

Keywords: brainsuite; glioblastoma; neural stem cells.

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Figures

Figure 1
Figure 1
Pre-operative cerebral MRI identified the centre (C), periphery (P) and marginal zone (MZ) of the tumour obtained during surgery.
Figure 2
Figure 2
Neurospheres formed in vitro by normal neural stem cells were detected in all the cultures established from C, P, and MZ of high-grade glioblastoma multiforme tumours.
Figure 3
Figure 3
C, P, and MZ cancer cells proliferated differently: at the first passage MZ cells proliferated more than C and P cells; at the second passage C, P, and MZ cells did not proliferate and at the third passage only C and P cells proliferated and the proliferation rate became exponentially higher for C cells.
Figure 4
Figure 4
Only MZ cells had the capacity to generate neurons, oligodendrocytes and astrocytes.
Figure 5
Figure 5
In our study, P cells expressed the highest percentage of CD133.
See this image and copyright information in PMC

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