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. 2014 Apr;29(2):213-20.
doi: 10.1007/s12291-013-0343-y. Epub 2013 Jun 5.

Anticancer Effect of dl-Glyceraldehyde and 2-Deoxyglucose in Ehrlich Ascites Carcinoma Bearing Mice and Their Effect on Liver, Kidney and Haematological Parameters

R Kapoor  1 , D B Gundpatil  1 , B L Somani  1 , T K Saha  1 , S Bandyopadhyay  2 , P Misra  1
Affiliations

Anticancer Effect of dl-Glyceraldehyde and 2-Deoxyglucose in Ehrlich Ascites Carcinoma Bearing Mice and Their Effect on Liver, Kidney and Haematological Parameters

R Kapoor et al. Indian J Clin Biochem. 2014 Apr.

Abstract

Cancer cells generally exhibit increased glycolysis for ATP generation (the Warburg effect). Compounds that inhibit glycolysis have potential applications in cancer treatment. dl-glyceraldehyde (DLG) and 2-Deoxyglucose (2-DG) have been proven effective in the inhibition of glucose metabolism. Ehrlich ascites carcinoma (EAC) cells were injected intraperitoneally (i.p) in 10-12 weeks old Swiss albino mice, weighing between 20 and 30 g. The anticancer activity of DLG and 2-DG were determined by tumor volume, tumor weight, viable and nonviable tumor cell count, average survival time, percentage increase in life span and tumor inhibition ratio. The blood samples were obtained for biochemical analysis after 9 days of treatment to study the effect on liver, kidney and haematological parameters. Histopathological examination of liver and kidney was also performed. One-way ANOVA test and Dunnett's test were used for comparisons of parameters in study groups. Both DLG and 2-DG individually decreased the tumor weight, tumor volume, viable tumor cell count and significantly increased the life span of treated mice, however the combination was found to be better. The biochemical parameters of liver and kidney functions and haematological parameters were restored close to control group as compared with the EAC bearing mice. Histopathological examination of liver and kidney in EAC control group showed large areas of necrosis, congestion and mononuclear cell infiltration but such changes were not observed in liver and kidney sections observed after i.p injection of DLG and 2-DG for 9 days. Improvement was much better in the group where combination of these two drugs were used.

Keywords: 2-Deoxyglucose; Anticancer; Ehrlich ascites carcinoma; Glycolysis; dl-Glyceraldehyde.

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Figures

Fig. 1
Fig. 1
Histopathological section of liver (40×) in EAC control mice (group II)
Fig. 2
Fig. 2
Histopathological section of liver (40×) in DLG and 2-DG treated mice (group V)
Fig. 3
Fig. 3
Histopathological section of kidney (40×) in EAC control mice (group II)
Fig. 4
Fig. 4
Histopathological section of Kidney (40×) in DLG and 2 DG treated mice (group V)
See this image and copyright information in PMC

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