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. 2014 Apr;20(2):241-7.
doi: 10.1007/s12298-014-0229-3. Epub 2014 Apr 3.

Synergistic effect of BAP and GA3 on in vitro flowering of Guizotia abyssinica Cass.-A multipurpose oil crop

S Baghel  1 , Y K Bansal  1
Affiliations

Synergistic effect of BAP and GA3 on in vitro flowering of Guizotia abyssinica Cass.-A multipurpose oil crop

S Baghel et al. Physiol Mol Biol Plants. 2014 Apr.

Abstract

Apical and axillary buds of Guizotia abyssinica Cass., isolated from seedlings raised in vitro, were cultured. High frequency of shoot regeneration was achieved on MS medium with BAP (1 mgl(-1)). Effect of BAP, Kn and GA3 applied successively in culture on shoot regeneration and flower bud formation has been studied. The shoots differentiated in cultures elongated on this medium. These rooted subsequently on half strength MS medium. The shoots flowered in vitro on MS medium with a combination of BAP (0.1mgl(-1)) + GA3 (0.1 mgl(-1)). The plantlets thus formed were successfully hardened with 90 % survival.

Keywords: Biodiesel; Guizotia abyssinica; In vitro flowering; Niger; Plant growth regulators.

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Figures

Fig. 1
Fig. 1
In vitro Regeneration and flowering in Guizotia abyssinica. 1–3 Natural Plant, Flower and Seed of Guizotia abyssinica. 4. Seed germination on Filter paper and Basal medium. 5. Shoot initiation with BAP (1mgl−1) 2 week. 6. Multiple shoot formation with BAP (1mgl−1) 3 week. 7–8. Multiple shoot formation with Kn (0.5 mgl−1). 9. Multiple shoot formation with BAP + GA3 (0.5 + 5 mgl−1) 3 week from Axillary explants. 10–11. Shoot elongation with Kn (0.5 mgl−1) 4 week from Apical and Axillary bud explants. 12–13. Flowering of in vitro regenerated plantlets. 14. Rooting of shoots in ½ strength MS medium. 15. Plantlets with well developed roots. 16. Hardened in vitro regenerated plants in poly cup. 17–18. Histological section showing shoot initiation and development. 19. Histological section showing multiple shoot formation. 20. Histological section showing carpel development 21. Histological section showing Pollen development. SAM shoot apical meristem, LP leaf primordium, VS provascular stand, MS multiple shoots, IF in vitro flower, FB flower bud, P pollen
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