doi: 10.1007/BF00291043.
5-Azadeoxycytidine induced undercondensation in the giant X chromosomes of Microtus agrestis
Affiliations
- PMID: 2476282
- DOI: 10.1007/BF00291043
Item in Clipboard
5-Azadeoxycytidine induced undercondensation in the giant X chromosomes of Microtus agrestis
Chromosoma.
1989 Aug.
Abstract
Fibroblasts of female Microtus agrestis were treated with 5-azadeoxycytidine (5-aza-dCyd) at a final concentration of 10(-5) M during the last 2 h of culture. This cytidine analogue induces distinct undercondensation of the constitutive heterochromatin in the giant X chromosomes. The undercondensed heterochromatic thread exhibits longitudinal segmentation reminiscent of a chromomere pattern. In the late-replicating X chromosome, 5-aza-dCyd also inhibits condensation of the genetically inactivated euchromatin (facultative heterochromatin). The described effects of 5-aza-dCyd on the X chromosome structure appear to be incorporation independent.
Similar articles
-
Inhibition of condensation in the late-replicating X chromosome induced by 5-azadeoxycytidine in human lymphocyte cultures.Hum Genet. 1988 May;79(1):18-23. doi: 10.1007/BF00291703. Hum Genet. 1988. PMID: 2452785
-
5-aza-C-induced changes in the time of replication of the X chromosomes of Microtus agrestis are followed by non-random reversion to a late pattern of replication.Chromosoma. 1987;95(1):81-8. doi: 10.1007/BF00293846. Chromosoma. 1987. PMID: 2438098
-
Transcriptional activity of constitutive heterochromatin in the mammal Microtus agrestis (Rodentia, Cricetidae).Exp Cell Res. 1987 Dec;173(2):463-72. doi: 10.1016/0014-4827(87)90286-2. Exp Cell Res. 1987. PMID: 2446895
-
Experimental condensation inhibition in constitutive and facultative heterochromatin of mammalian chromosomes.Cytogenet Cell Genet. 2000;91(1-4):113-23. doi: 10.1159/000056830. Cytogenet Cell Genet. 2000. PMID: 11173842
-
The effects of 5-azacytidine and 5-azadeoxycytidine on chromosome structure and function: implications for methylation-associated cellular processes.Pharmacol Ther. 1995 Jan;65(1):19-46. doi: 10.1016/0163-7258(94)00053-6. Pharmacol Ther. 1995. PMID: 7536332 Review.
Cited by
-
Differential digestion of the centromeric heterochromatic regions of the 5-azacytidine-decondensed human chromosomes 1, 9, 15, and 16 by NdeII and Sau3AI restriction endonucleases.Genetica. 1995;96(3):235-8. doi: 10.1007/BF01439577. Genetica. 1995. PMID: 8522163
-
Challenges and Opportunities for Clinical Cytogenetics in the 21st Century.Genes (Basel). 2023 Feb 15;14(2):493. doi: 10.3390/genes14020493. Genes (Basel). 2023. PMID: 36833419 Free PMC article.
-
Cytosine methylation associated with repeat-induced point mutation causes epigenetic gene silencing in Neurospora crassa.Genetics. 1997 Jun;146(2):509-23. doi: 10.1093/genetics/146.2.509. Genetics. 1997. PMID: 9178002 Free PMC article.
-
The Importance of Monitoring Non-clonal Chromosome Aberrations (NCCAs) in Cancer Research.Methods Mol Biol. 2024;2825:79-111. doi: 10.1007/978-1-0716-3946-7_4. Methods Mol Biol. 2024. PMID: 38913304
-
Toxicity of 5-aza-2'-deoxycytidine to mammalian cells is mediated primarily by covalent trapping of DNA methyltransferase rather than DNA demethylation.Proc Natl Acad Sci U S A. 1994 Dec 6;91(25):11797-801. doi: 10.1073/pnas.91.25.11797. Proc Natl Acad Sci U S A. 1994. PMID: 7527544 Free PMC article.