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. 1989 Sep;86(18):6925-9.
doi: 10.1073/pnas.86.18.6925.

Human immunodeficiency virus 1 tat protein binds trans-activation-responsive region (TAR) RNA in vitro

C Dingwall  1 , I Ernberg, M J Gait, S M Green, S Heaphy, J Karn, A D Lowe, M Singh, M A Skinner, R Valerio
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Human immunodeficiency virus 1 tat protein binds trans-activation-responsive region (TAR) RNA in vitro

C Dingwall et al. Proc Natl Acad Sci U S A. 1989 Sep.

Abstract

tat, the trans-activator protein for human immunodeficiency virus 1 (HIV-1), has been expressed in Escherichia coli from synthetic genes. Purified tat binds specifically to HIV-1 trans-activation-responsive region (TAR) RNA in gel-retardation, filter-binding, and immunoprecipitation assays. tat does not bind detectably to antisense TAR RNA sequences, cellular mRNA sequences, variant TAR RNA sequences with altered stem-loop structures, or TAR DNA.

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References

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