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. 1989 Sep 22;245(4924):1396-9.
doi: 10.1126/science.2476851.

Sequence-specific isotope effects on the cleavage of DNA by bleomycin

J W Kozarich  1 , L Worth Jr, B L Frank, D F Christner, D E Vanderwall, J Stubbe
Affiliations

Sequence-specific isotope effects on the cleavage of DNA by bleomycin

J W Kozarich et al. Science. .

Abstract

Bleomycin is a metal- and oxygen-dependent DNA cleaver. The chemistry of DNA damage has been proposed to involve rate-limiting abstraction of the 4'-hydrogen. A DNA fragment has been prepared that contains [4'-2H]thymidine residues of high isotopic content. Primary kinetic isotope effects have been directly observed at individual thymidine residues with DNA sequencing technology.

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