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. 2014 Aug 15;30(16):2389-90.
doi: 10.1093/bioinformatics/btu202. Epub 2014 Apr 25.

ThunderSTORM: a comprehensive ImageJ plug-in for PALM and STORM data analysis and super-resolution imaging

Martin Ovesný  1 , Pavel Křížek  1 , Josef Borkovec  1 , Zdeněk Svindrych  1 , Guy M Hagen  1
Affiliations

ThunderSTORM: a comprehensive ImageJ plug-in for PALM and STORM data analysis and super-resolution imaging

Martin Ovesný et al. Bioinformatics. .

Abstract

ThunderSTORM is an open-source, interactive and modular plug-in for ImageJ designed for automated processing, analysis and visualization of data acquired by single-molecule localization microscopy methods such as photo-activated localization microscopy and stochastic optical reconstruction microscopy. ThunderSTORM offers an extensive collection of processing and post-processing methods so that users can easily adapt the process of analysis to their data. ThunderSTORM also offers a set of tools for creation of simulated data and quantitative performance evaluation of localization algorithms using Monte Carlo simulations.

Availability and implementation: ThunderSTORM and the online documentation are both freely accessible at https://code.google.com/p/thunder-storm/.

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Figures

Fig. 1.
Fig. 1.
Simulations and SMLM reconstruction with ThunderSTORM. (a) Example of a mask used for generating simulated SMLM data. The gray-scale intensity values are interpreted as molecular densities within a user-specified range. (b) SMLM reconstruction of a simulated dataset. (c) Detail of b. (d) Widefield fluorescence image of an A431 epidermoid carcinoma cell expressing the membrane protein mCitrine-erbB3. (e) SMLM reconstruction using the default settings. (f) Detail of e. SMLM imaging was performed as previously described (Křížek et al., 2011)
See this image and copyright information in PMC

References

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