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Review
. 2014 Jun;20(3):215-21.
doi: 10.1089/mdr.2014.0032. Epub 2014 Apr 28.

The elongation of ovococci

Jules Philippe  1 , Thierry Vernet, André Zapun
Affiliations
Review

The elongation of ovococci

Jules Philippe et al. Microb Drug Resist. 2014 Jun.

Abstract

The morphogenesis of ovococci has been reviewed extensively. Recent results have provided new insights concerning the mechanisms of elongation in ovoid bacteria. We present here the proteins involved in the elongation (firmly established and more or less hypothetical) and discuss the relationship between elongation and division of ovococci.

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Figures

<b>FIG. 1.</b>
FIG. 1.
Topology of the elongation proteins in ovococci. The implication of MreC, MreD, PBP2b,, and RodA in elongation was experimentally shown in ovococci. The implication of PBP1a and RodZ is hypothetical.
<b>FIG. 2.</b>
FIG. 2.
Isolation of recombinant MreC/MreD and PBP2b/RodA complexes from pneumococcus. (A) MreC and MreD were expressed in Escherichia coli from an artificial operon fused with N-terminal Strep- and His8-tags, respectively. Membranes were isolated and solubilized with n-dodecyl-β-d-maltopyranoside. The complex was isolated by two successive affinity chromatography steps on Strep-Tactin® and Ni-NTA. (B) PBP2b with a C-terminal Strep-tag and RodA with an N-terminal His8-tag were similarly expressed and the complex isolated, in that case, first by Ni-NTA chromatography followed by a Strep-Tactin® purification. L, W, and E stand for load, wash, and elution. Samples were analyzed by Coomassie-stained sodium dodecyl sulfate–polyacrylamide gel electrophoresis.
See this image and copyright information in PMC

References

    1. Alyahya S.A., Alexander R., Costa T.Henriques A.O.Emonet T., and Jacobs-Wagner C. 2009. RodZ, a component of the bacterial core morphogenic apparatus. Proc. Natl Acad. Sci. U. S. A. 106:1239–1244 - PMC - PubMed
    1. Bendezu F.O., Hale C.A.Bernhardt T.G. and de Boer P.A. 2009. RodZ (YfgA) is required for proper assembly of the MreB actin cytoskeleton and cell shape in E. coli. EMBO J. 28:193–204 - PMC - PubMed
    1. Berg K.H., Stamsas G.A., Straume D., and Havarstein L.S. 2013. Effects of low PBP2b levels on cell morphology and peptidoglycan composition in Streptococcus pneumoniae R6. J. Bacteriol. 195:4342–4354 - PMC - PubMed
    1. Bui N.K., Eberhardt A., Vollmer D., Kern T., Bougault C., Tomasz A.Simorre J.P. and Vollmer W. 2012. Isolation and analysis of cell wall components from Streptococcus pneumoniae. Anal. Biochem. 421:657–666 - PubMed
    1. Claessen D., Emmins R.Hamoen L.W.Daniel R.A.Errington J., and Edwards D.H. 2008. Control of the cell elongation-division cycle by shuttling of PBP1 protein in Bacillus subtilis. Mol. Microbiol. 68:1029–1046 - PubMed

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