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. 1989 Oct 11;17(19):7879-89.
doi: 10.1093/nar/17.19.7879.

Catalytic activity is retained in the Tetrahymena group I intron despite removal of the large extension of element P5

G F Joyce  1 , G van der Horst, T Inoue
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Free PMC article

Catalytic activity is retained in the Tetrahymena group I intron despite removal of the large extension of element P5

G F Joyce et al. Nucleic Acids Res. .
Free PMC article

Abstract

We have made sizeable internal deletions within the self-splicing group I intron of Tetrahymena thermophila. Deletions were made in a piecewise manner in order to remove secondary structural elements thought to be extraneous to the catalytic center of the molecule. The resulting deletion mutants retain self-splicing activity, albeit under modified reaction conditions that enhance duplex stability. Considering those portions of the molecule that can be deleted without a loss of catalytic activity, one is left with a catalytic center of approximately 130 nucleotides that is solely responsible for the molecule's activity.

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References

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