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Comparative Study
. 2014 May;27(4):174-8.
doi: 10.1089/vim.2013.0111. Epub 2014 Apr 30.

Turkey versus guinea pig red blood cells: hemagglutination differences alter hemagglutination inhibition responses against influenza A/H1N1

Inna G Ovsyannikova  1 , Sarah J White, Randy A Albrecht, Adolfo García-Sastre, Gregory A Poland
Affiliations
Comparative Study

Turkey versus guinea pig red blood cells: hemagglutination differences alter hemagglutination inhibition responses against influenza A/H1N1

Inna G Ovsyannikova et al. Viral Immunol. 2014 May.

Abstract

For influenza hemagglutination inhibition (HAI) assays, species selection of red blood cells (RBCs) is critical to determine antibody titers to influenza viruses reliably. We compared pandemic influenza virus A/H1N1 (pdH1N1) HAI titers using turkey or guinea pig RBCs. Turkey RBCs appear to be the more appropriate species choice for influenza A/pH1N1 HAI assays.

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Figures

<b>FIG. 1.</b>
FIG. 1.
A comparison of hemagglutination inhibition (HAI) titers against the H1N1 influenza vaccine strain obtained with turkey or guinea pig red blood cells (RBCs) in an older adult population (percent). The dotted lines represent the lowest level of seroprotection (1:40 HAI antibody titer).
See this image and copyright information in PMC

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