The expression of sequences similar to the human c-erb-A oncogene are regulated in a tissue and stage specific manner in Drosophila melanogaster

Abstract

The gene encoding the human thyroid hormone receptor, c-erb-A, is hypothesized to be a member of a superfamily of genes, which originated from a primordial receptor encoding gene. To trace the molecular evolutionary background of this gene, we initiated the search for it in the genome of Drosophila melanogaster by using low stringency hybridization analysis. We report here the presence of more than one gene in Drosophila with varying degrees of sequence similarity. By RNA blot hybridization analyses utilizing both wild type and a temperature sensitive ecdysone deficient mutant strain, ecd1, with a human c-erb-A probe, we show that a single high molecular weight RNA of 6.8kb in size is recognized by human c-erb-A, is regulated in a stage and tissue specific manner, and is also inducible in organisms of the ecd1 mutant strain in the presence of exogeneous ecdysone.