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. 2014 Apr 18;7(2):256-63.
doi: 10.3980/j.issn.2222-3959.2014.02.12. eCollection 2014.

Comparative proteomic analysis of plasma proteins in patients with age-related macular degeneration

Xin-Rong Xu  1 , Lu Zhong  1 , Bing-Lin Huang  1 , Yuan-Hua Wei  2 , Xin Zhou  1 , Ling Wang  3 , Fu-Qiang Wang  3
Affiliations

Comparative proteomic analysis of plasma proteins in patients with age-related macular degeneration

Xin-Rong Xu et al. Int J Ophthalmol. .

Abstract

Aim: To find the significant altered proteins in age-related macular degeneration (AMD) patients as potential biomarkers of AMD.

Methods: A comparative analysis of the protein pattern of AMD patients versus healthy controls was performed by means of proteomic analysis using two-dimensional gel electrophoresis followed by protein identification with MALDI TOF/TOF mass spectrometry.

Results: We identified 28 proteins that were significantly altered with clinical relevance in AMD patients. These proteins were involved in a wide range of biological functions including immune responses, growth cytokines, cell fate determination, wound healing, metabolism, and anti-oxidance.

Conclusion: These results demonstrate the capacity of proteomic analysis of AMD patient plasma. In addition to the utility of this approach for biomarker discovery, identification of alterations in endogenous proteins in the plasma of AMD patient could improve our understanding of the disease pathogenesis.

Keywords: age-related macular degeneration; clinical patients; comparative proteomics; plasma proteins; two-dimensional electrophoresis.

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Figures

Figure 1
Figure 1. Two-dimensional electrophoresis analyses of plasma proteins in patients with AMD
A: Healthy control subjects; B: Dry AMD patients; C: Wet AMD patients. Precipitations were performed as described in the section of Subjects and Methods. Two-dimensional analysis was performed using 100 µg of protein, in pH 3-10 IPG strips (18 cm) in the first dimension and 12.5% SDS gels in the second dimension. The gels were stained using glutaraldehyde silver staining method. Representative blots from all tested samples are shown.
Figure 2
Figure 2. Two-dimensional electrophoresis analyses of plasma proteins in 6 healthy subjects. The matching rate for the protein spots was 91.5%.
Figure 3
Figure 3. Two-dimensional electrophoresis analyses of plasma proteins in 11 patients with dry AMD. The matching rate for the protein spots was 92.2%.
Figure 4
Figure 4. Two-dimensional electrophoresis analyses of plasma proteins in 13 patients with wet AMD. The matching rate for the protein spots was 92.9%.
See this image and copyright information in PMC

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