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. 2014 Jun;11(6):633-6.
doi: 10.1038/nmeth.2940. Epub 2014 May 4.

Reversible protein inactivation by optogenetic trapping in cells

Sangkyu Lee  1 , Hyerim Park  2 , Taeyoon Kyung  3 , Na Yeon Kim  3 , Sungsoo Kim  3 , Jihoon Kim  3 , Won Do Heo  4
Affiliations

Reversible protein inactivation by optogenetic trapping in cells

Sangkyu Lee et al. Nat Methods. 2014 Jun.

Abstract

We present a versatile platform to inactivate proteins in living cells using light, light-activated reversible inhibition by assembled trap (LARIAT), which sequesters target proteins into complexes formed by multimeric proteins and a blue light-mediated heterodimerization module. Using LARIAT, we inhibited diverse proteins that modulate cytoskeleton, lipid signaling and cell cycle with high spatiotemporal resolution. Use of single-domain antibodies extends the method to target proteins containing specific epitopes, including GFP.

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