Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 May;15(5):482-90.
doi: 10.1631/jzus.B1300203.

Adipose-derived stem cells transfected with pEGFP-OSX enhance bone formation during distraction osteogenesis

Qing-guo Lai  1 , Shao-long Sun, Xiao-hong Zhou, Chen-ping Zhang, Kui-feng Yuan, Zhong-jun Yang, Sheng-lei Luo, Xiao-peng Tang, Jiang-bo Ci
Affiliations

Adipose-derived stem cells transfected with pEGFP-OSX enhance bone formation during distraction osteogenesis

Qing-guo Lai et al. J Zhejiang Univ Sci B. 2014 May.

Abstract

This study was designed to investigate the effects of local delivery of adipose-derived stem cells (ADSCs) transfected with transcription factor osterix (OSX) on bone formation during distraction osteogenesis. New Zealand white rabbits (n=54) were randomly divided into three groups (18 rabbits per group). A directed cloning technique was used for the construction of recombinant plasmid pEGFP-OSX, where EGFP is the enhanced green fluorescence protein. After osteodistraction of the right mandible of all experimental rabbits, rabbits in group A were treated with ADSCs transfected with pEGFP-OSX, group B with ADSCs transfected with pEGFP-N1, and group C with physiological saline. Radiographic and histological examinations were processed after half of the animals within each group were humanely killed by injection of sodium pentothal at Week 2 or 6 after surgery. The distraction bone density was measured as its projectional bone mineral density (BMD). Three parameters were measured, namely, the thickness of new trabeculae (TNT), and the volumes of the newly generated cortical bone (NBV1) and the cancellous bone (NBV2) of the distracted regions. Good bone generation in the distraction areas was found in group A, which had the highest BMD, TNT, and NBV in the distraction zones among the groups. There was no significant difference in bone generation in the distraction areas between groups B and C. The results indicate that the transplantation of ADSCs transfected with pEGFP-OSX can effectively promote bone generation during distraction in vivo.

Keywords: Adipose-derived stem cell (ADSC); Distraction osteogenesis; Gene transfection; Transcription factor.

PubMed Disclaimer

Conflict of interest statement

Compliance with ethics guidelines: Qing-guo LAI, Shao-long SUN, Xiao-hong ZHOU, Chen-ping ZHANG, Kui-feng YUAN, Zhong-jun YANG, Sheng-lei LUO, Xiao-peng TANG, and Jiang-bo CI declare that they have no conflict of interest.

All institutional and national guidelines for the care and use of laboratory animals were followed.

Figures

Fig. 1
Fig. 1
Custom-made internal distractor purchased from Xi’an Zhongbang Titanium Biological Materials Co., Ltd., China
Fig. 2
Fig. 2
Custom-made distraction device fixed in situ
Fig. 3
Fig. 3
Lateral radiographs of distracted mandibles in three groups at Weeks 2 and 6 after the end of distraction
Fig. 4
Fig. 4
Radiodensity of distracted gaps of different groups at Weeks 2 and 6 * P<0.01 vs. group A. Data are expressed as mean±SD (n=9)
Fig. 5
Fig. 5
Histological behavior of the distracted bone at Weeks 2 and 6 in rabbit mandibles (H&E staining) (a, d) Group A; (b, e) Groups B; (c, f) Group C
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Asahara T, Kalka C, Isner JM. Stem cell therapy and gene transfer for regeneration. Gene Ther. 2000;7(6):451–457. doi: 10.1038/sj.gt.3301142. - DOI - PubMed
    1. Ashinoff RL, Cetrulo CL, Jr, Galiano RD, et al. Bone morphogenic protein-2 gene therapy for mandibular distraction osteogenesis. Ann Plast Surg. 2004;52(6):585–590. doi: 10.1097/01.sap.0000123023.28874.1e. - DOI - PubMed
    1. Banfi A, Bianchi G, Galotto M, et al. Bone marrow stromal damage after chemo/radiotherapy: occurrence, consequences and possibilities of treatment. Leuk Lymphoma. 2001;42(5):863–870. doi: 10.3109/10428190109097705. - DOI - PubMed
    1. Banfi A, Podestà M, Fazzuoli L, et al. High-dose chemotherapy shows a dose-dependent toxicity to bone marrow osteoprogenitors: a mechanism for post-bone marrow transplantation osteopenia. Cancer. 2001;92(9):2419–2428. doi: 10.1002/1097-0142(20011101)92:9<2419::AID-CNCR1591>3.0.CO;2-K. - DOI - PubMed
    1. Breitbart AS, Grande DA, Mason JM, et al. Gene-enhanced tissue engineering: applications for bone healing using cultured periosteal cells transduced retrovirally with the BMP-7 gene. Ann Plast Surg. 1999;42(5):488–495. doi: 10.1097/00000637-199905000-00005. - DOI - PubMed

Publication types

MeSH terms

Substances