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Comment
. 2014 May 13;111(19):6860-1.
doi: 10.1073/pnas.1405194111. Epub 2014 May 5.

Measuring reversal of HIV-1 latency ex vivo using cells from infected individuals

Ya-Chi Ho  1 , Gregory M Laird  1 , Robert F Siliciano  2
Affiliations
Comment

Measuring reversal of HIV-1 latency ex vivo using cells from infected individuals

Ya-Chi Ho et al. Proc Natl Acad Sci U S A. .
No abstract available

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig. 1.
Fig. 1.
Ex vivo measurement of HIV-1 latency reversal. (A) Patient resting CD4+ T cells cultured ex vivo with anti-CD3/CD28 costimulation, vorinostat, or media control. Although the majority of the HIV-1 proviruses are not induced by these stimuli (blue), a small fraction of the proviruses can be transcribed on stimulation, as detected by measurement of cell-associated HIV-1 RNAs (magenta). Even fewer proviruses can produce viral particles as detected by supernatant viral RNA (yellow). (B) Comparison of supernatant viral RNA (yellow), cell-associated RNA (magenta), and total proviral DNA (blue) after stimulation with anti-CD3/CD28, vorinostat, or media control. Percentage represents fractional provirus expression (fPVE) as defined by Cillo et al. (11).
See this image and copyright information in PMC

Comment on

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