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. 2014:2014:527393.
doi: 10.1155/2014/527393. Epub 2014 Apr 3.

A comparative study of phase States of the peribacteroid membrane from yellow lupin and broad bean nodules

Natalia N Kudryavtseva  1 , Alexis V Sof'in  1 , Georgiy S Bobylev  2 , Evgeny M Sorokin  2
Affiliations

A comparative study of phase States of the peribacteroid membrane from yellow lupin and broad bean nodules

Natalia N Kudryavtseva et al. Biochem Res Int. 2014.

Abstract

A comparative study of the lipid bilayer phase status and structure of the outer membrane of free-living Bradyrhizobium strain 359a (Nod(+)Fix(+)) and 400 (Nod(+)FixL) or Rhizobium leguminosarum 97 (Nod(+)Fix(+), effective) and 87 (Nod(+)FixL, ineffective) has been carried out. Also, the effect of the symbiotic pair combination on the lipid bilayer structure of the bacteroid outer membrane and peribacteroid membrane, isolated from the nodules of Lupinus luteus L. or Vicia faba L., has been studied. As a result, it is shown that the lipid bilayer status of the bacteroid outer membrane is mainly determined by microsymbiont, but not the host plant. In the contrast, the lipid bilayer status of the peribacteroid membrane and, as a consequence, its properties depend on interaction of both symbiotic partners.

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Figures

Figure 1
Figure 1
The temperature dependence of the steady state fluorescence anisotropy (r) of 1,6-diphenyl-1,3,5-hexatriene (DPH) embedded in a lipid matrix of the bacterial outer membrane of the free-living B. lupini strains 359a (1) and 400 (3) as well as isolated bacteroids formed by these strains (2 and 4, resp.).
Figure 2
Figure 2
The temperature dependence of the steady state fluorescence anisotropy (r) of 1,6-diphenyl-1,3,5-hexatriene (DPH) embedded in a lipid matrix of the bacterial outer membrane of the free-living R. leguminosarum strains 97 (1) and 87 (3) as well as isolated bacteroids formed by these strains (2 and 4, resp.).
Figure 3
Figure 3
The temperature dependence of the steady state fluorescence anisotropy (r) of 1,6-diphenyl-1,3,5-hexatriene (DPH) embedded in a lipid matrix of the plasmalemma specimens from Lupinus luteus L. (1) as well as the peribacteroid membrane specimens isolated from the lupin nodules, formed by the B. lupini strains 359a (2) and 400 (3).
Figure 4
Figure 4
The temperature dependence of the steady state fluorescence anisotropy (r) of 1,6-diphenyl-1,3,5-hexatriene (DPH) embedded in a lipid matrix of the peribacteroid membrane specimens isolated from the broad bean Vicia faba L. nodules, formed by the R. leguminosarum strains 97 (1) and 87 (2).
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