Kinetics of histamine released from rectal mucosa

Abstract

This kinetic study was performed to investigate the different tissue-influencing histamine amounts in Crohn's Disease (CD), Ulcerative Colitis (UC), patients with polyps and cancers (P.a.C.Gr) and in a Control Group (CG). For this purpose the endoscopically obtained specimens from rectal mucosa were immediately placed into 1000 microliters of Hank's incubation medium in order to determine the spontaneously released histamine amounts at the time points of 5, 10, 15, 20 and 30 minutes. Each time a volume of 100 microliters was removed from the incubation medium and the kinetic value (KV) was detected by using the single isotope radioenzymatic method. Influencing of natural histamine catabolism and the comparison of the tissue histamine release with or without air oxygen in the incubation medium using four kinetic programmes (KP1-4) provides clearly different KVs, not only between the KPs but also within the same KP. The P.a.C.Gr. shows higher kinetic values (KVs) compared with the CG. In KP1-3 the kinetic courses (KCs) of the Inflammatory Bowel Diseases (IBDs), CD and UC-both not yet divided in active (a.) or not active (n.a.) disease stages-cross the KCs of the CG several times. Only the differentiation of the IBDs in active and not active disease stages in KP4 reveals that CDa. and UCa. stand out from the CG by higher KVs, and in contrast, CDn.a. and UCn.a. have lower KVs than the CG. The released amounts of histamine in CDa. and UCa. are significantly higher than in CDn.a. and UCn.a.