Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL

Kunio Hirata¹, Kyoko Shinzawa-Itoh², Naomine Yano³, Shuhei Takemura⁴, Koji Kato², Miki Hatanaka⁴, Kazumasa Muramoto⁴, Takako Kawahara⁴, Tomitake Tsukihara⁵, Eiki Yamashita⁶, Kensuke Tono⁷, Go Ueno⁸, Takaaki Hikima⁸, Hironori Murakami⁸, Yuichi Inubushi⁸, Makina Yabashi⁸, Tetsuya Ishikawa⁸, Masaki Yamamoto⁸, Takashi Ogura⁹, Hiroshi Sugimoto⁸, Jian-Ren Shen¹⁰, Shinya Yoshikawa⁴, Hideo Ago⁸

Affiliations

¹ 1] RIKEN SPring-8 Center, Sayo, Japan. [2] Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CREST), Kawaguchi, Japan. [3].
² 1] Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan. [2].
³ 1] Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CREST), Kawaguchi, Japan. [2] Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan.
⁴ Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan.
⁵ 1] Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CREST), Kawaguchi, Japan. [2] Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan. [3] Institute for Protein Research, Osaka University, Suita, Japan.
⁶ Institute for Protein Research, Osaka University, Suita, Japan.
⁷ Japan Synchrotron Radiation Research Institute, Sayo, Japan.
⁸ RIKEN SPring-8 Center, Sayo, Japan.
⁹ Picobiology Institute, Graduate School of Life Science, University of Hyogo, Sayo, Japan.
¹⁰ Photosynthesis Research Center, Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan.

PMID: 24813624
DOI: 10.1038/nmeth.2962

Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL

Kunio Hirata et al. Nat Methods. 2014 Jul.

. 2014 Jul;11(7):734-6.

doi: 10.1038/nmeth.2962. Epub 2014 May 11.

Authors

Affiliations

¹ 1] RIKEN SPring-8 Center, Sayo, Japan. [2] Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CREST), Kawaguchi, Japan. [3].
² 1] Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan. [2].
³ 1] Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CREST), Kawaguchi, Japan. [2] Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan.
⁴ Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan.
⁵ 1] Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CREST), Kawaguchi, Japan. [2] Picobiology Institute, Graduate School of Life Science, University of Hyogo, Kamigori-cho, Japan. [3] Institute for Protein Research, Osaka University, Suita, Japan.
⁶ Institute for Protein Research, Osaka University, Suita, Japan.
⁷ Japan Synchrotron Radiation Research Institute, Sayo, Japan.
⁸ RIKEN SPring-8 Center, Sayo, Japan.
⁹ Picobiology Institute, Graduate School of Life Science, University of Hyogo, Sayo, Japan.
¹⁰ Photosynthesis Research Center, Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan.

PMID: 24813624
DOI: 10.1038/nmeth.2962

Abstract

We report a method of femtosecond crystallography for solving radiation damage-free crystal structures of large proteins at sub-angstrom spatial resolution, using a large single crystal and the femtosecond pulses of an X-ray free-electron laser (XFEL). We demonstrated the performance of the method by determining a 1.9-Å radiation damage-free structure of bovine cytochrome c oxidase, a large (420-kDa), highly radiation-sensitive membrane protein.

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References

1. Proc Natl Acad Sci U S A. 2009 Feb 17;106(7):2165-9 - PubMed
1. Nature. 2011 May 5;473(7345):55-60 - PubMed
1. Annu Rev Biophys. 2011;40:205-23 - PubMed
1. Science. 1987 Jan 23;235(4787):458-60 - PubMed
1. Methods Enzymol. 1997;276:307-26 - PubMed

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Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL

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Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL

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