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. 2014:1145:167-89.
doi: 10.1007/978-1-4939-0446-4_14.

In vitro culture for doubled haploids: tools for molecular breeding

Sue Broughton  1 , Parminder K Sidhu, Philip A Davies
Affiliations

In vitro culture for doubled haploids: tools for molecular breeding

Sue Broughton et al. Methods Mol Biol. 2014.

Abstract

Doubled haploid (DH) techniques using immature microspores or embryos rescued in vitro following interspecific hybridization are important tools for breeding wheat and barley and for generating populations for gene mapping purposes. Here, we describe methods being used for the successful production of spring wheat and barley DHs in Australia.

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