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. 2014 Mar 15;7(4):1733-4.
eCollection 2014.

Staphylococcus pseudintermedius for CAMP-test

Vincenzo Savini  1 , Antonello Paparella  2 , Annalisa Serio  2 , Roberta Marrollo  1 , Edoardo Carretto  3 , Paolo Fazii  1
Affiliations

Staphylococcus pseudintermedius for CAMP-test

Vincenzo Savini et al. Int J Clin Exp Pathol. .

Abstract

CAMP test reliably detects Listeria monocytogenes (Lm) and Streptococcus agalactiae (group B streptococcus, GBS); it is traditionally performed streaking the tested isolate perpendicularly to Staphylococcus aureus (Sa), provided that reference Sa strains (that produce β-hemolysin) are used. In a zone of β-hemolysin activity, in fact, GBS and Lm form typical arrow-shaped hemolytic areas. While Sa production of the toxin is strain-dependent, however, that of Staphylococcus pseudintermedius (Sp), a pet-owner colonizer and an emerging human pathogen, is constitutive, then observed in all clinical isolates. Therefore, Sp may indeed represent a valid alternative to perform the assay.

Keywords: CAMP-test; GBS; Listeria monocytogenes; Staphylococcus pseudintermedius; β-hemolysin.

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Figures

Figure 1
Figure 1
positive CAMP test: top, L. monocytogenes inoculum - bottom, GBS (nonhemolytic variant) inoculum - middle (horizontal inoculum) S. pseudintermedius inoculum. The test is performed on Tryptic Soy agar (TSS, Liofilchem®).
Figure 2
Figure 2
L. monocytogenes grown on Liofilchem® O.A. Listeria Agar (typical halos are visible around blue-green colonies).
See this image and copyright information in PMC

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